UNIGE document Scientific Article
previous document  unige:74743  next document
add to browser collection
Title

'98 Escherichia coli SWISS-2DPAGE database update

Authors
Walsh, Brand J
Ou, Keli
Tyler, Margaret
Gooley, Andrew A
show hidden authors show all authors [1 - 17]
Published in Electrophoresis. 1998, vol. 19, no. 11, p. 1960-71
Abstract The combination of two-dimensional polyacrylamide gel electrophoresis (2-D PAGE), computer image analysis and several protein identification techniques allowed the Escherichia coli SWISS-2DPAGE database to be established. This is part of the ExPASy molecular biology server accessible through the WWW at the URL address http://www.expasy.ch/ch2d/ch2d-top.html . Here we report recent progress in the development of the E. coli SWISS-2DPAGE database. Proteins were separated with immobilized pH gradients in the first dimension and sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the second dimension. To increase the resolution of the separation and thus the number of identified proteins, a variety of wide and narrow range immobilized pH gradients were used in the first dimension. Micropreparative gels were electroblotted onto polyvinylidene difluoride membranes and spots were visualized by amido black staining. Protein identification techniques such as amino acid composition analysis, gel comparison and microsequencing were used, as well as a recently described Edman "sequence tag" approach. Some of the above identification techniques were coupled with database searching tools. Currently 231 polypeptides are identified on the E. coli SWISS-2DPAGE map: 64 have been identified by N-terminal microsequencing, 39 by amino acid composition, and 82 by sequence tag. Of 153 proteins putatively identified by gel comparison, 65 have been confirmed. Many proteins have been identified using more than one technique. Faster progress in the E. coli proteome project will now be possible with advances in biochemical methodology and with the completion of the entire E. coli genome.
Keywords Bacterial Proteins/analysisDatabases, FactualElectrophoresis, Gel, Two-DimensionalEscherichia coli/chemistryIsoelectric PointMembranes, ArtificialPolyvinylsStaining and Labeling/methods
Identifiers
PMID: 9740056
Full text
Article (Published version) (1.3 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research groups Calipho (80)
Groupe de Protéomique biomédicale (635)
Citation
(ISO format)
TONELLA, Luisa et al. '98 Escherichia coli SWISS-2DPAGE database update. In: Electrophoresis, 1998, vol. 19, n° 11, p. 1960-71. https://archive-ouverte.unige.ch/unige:74743

215 hits

1 download

Update

Deposited on : 2015-08-18

Export document
Format :
Citation style :