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Scientific article
English

Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice

Published inEuropean Journal of Immunology, vol. 29, no. 10, p. 3390-3400
Publication date1999
Abstract

Early life responses to respiratory syncytial virus (RSV)-F DNA and RSV-F protein immunization were studied in murine models of neonatal immunization. RSV-F DNA induced similar antibody (Ab) responses, antigen-specific IFN-gamma production and cytotoxic T lymphocyte (CTL) responses in 1-week-old and adult BALB / c mice. In contrast, RSV-F protein induced much higher IL-5 responses in early life. Both vaccines elicited Ab and CTL responses in spite of maternal Ab, but with distinctive kinetics. Sequential RSV-F DNA priming / protein boosting primed 1-week-old mice for RSV-F-specific CTL responses, reduced IL-5 production and enhanced Ab responses. In contrast, IL-5 exceeded IFN-gamma responses when young mice were primed with protein and boosted with DNA. Last, when protein and DNA immunization were combined, a single vaccine dose induced early Ab responses, preferential IL-5 responses but strong CTL responses. Sequential or combined DNA / protein immunization thus represent interesting strategies for early life immunization.

Keywords
  • Animals
  • HN Protein
  • Immunity, Maternally-Acquired
  • Immunoglobulin G/biosynthesis
  • Mice
  • Mice, Inbred BALB C
  • Respiratory Syncytial Virus Infections/prevention & control
  • Respiratory Syncytial Viruses/immunology
  • T-Lymphocytes, Cytotoxic/immunology
  • Th1 Cells/immunology
  • Time Factors
  • Vaccines, Combined/immunology
  • Vaccines, DNA/immunology
  • Viral Envelope Proteins
  • Viral Proteins/immunology
  • Viral Vaccines/immunology
Citation (ISO format)
MARTINEZ, Javier et al. Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice. In: European Journal of Immunology, 1999, vol. 29, n° 10, p. 3390–3400. doi: 10.1002/(SICI)1521-4141(199910)29:10<3390::AID-IMMU3390>3.0.CO;2-A
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Article (Published version)
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ISSN of the journal0014-2980
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