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Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice

Published in European Journal of Immunology. 1999, vol. 29, no. 10, p. 3390-400
Abstract Early life responses to respiratory syncytial virus (RSV)-F DNA and RSV-F protein immunization were studied in murine models of neonatal immunization. RSV-F DNA induced similar antibody (Ab) responses, antigen-specific IFN-gamma production and cytotoxic T lymphocyte (CTL) responses in 1-week-old and adult BALB / c mice. In contrast, RSV-F protein induced much higher IL-5 responses in early life. Both vaccines elicited Ab and CTL responses in spite of maternal Ab, but with distinctive kinetics. Sequential RSV-F DNA priming / protein boosting primed 1-week-old mice for RSV-F-specific CTL responses, reduced IL-5 production and enhanced Ab responses. In contrast, IL-5 exceeded IFN-gamma responses when young mice were primed with protein and boosted with DNA. Last, when protein and DNA immunization were combined, a single vaccine dose induced early Ab responses, preferential IL-5 responses but strong CTL responses. Sequential or combined DNA / protein immunization thus represent interesting strategies for early life immunization.
Keywords AnimalsHN ProteinImmunity, Maternally-AcquiredImmunoglobulin G/biosynthesisMiceMice, Inbred BALB CRespiratory Syncytial Virus Infections/prevention & controlRespiratory Syncytial Viruses/immunologyT-Lymphocytes, Cytotoxic/immunologyTh1 Cells/immunologyTime FactorsVaccines, Combined/immunologyVaccines, DNA/immunologyViral Envelope ProteinsViral Proteins/immunologyViral Vaccines/immunology
PMID: 10540351
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Research group Centre de Vaccinologie et d'Immunologie néonatale (177)
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MARTINEZ, Javier et al. Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice. In: European Journal of Immunology, 1999, vol. 29, n° 10, p. 3390-400. doi: 10.1002/(SICI)1521-4141(199910)29:10<3390::AID-IMMU3390>3.0.CO;2-A https://archive-ouverte.unige.ch/unige:74353

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Deposited on : 2015-07-27

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