Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice

Article Article scientifique Article Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice eng 1999 author Martinez Javier 87672 author Lin Xiao 729053 author Kovarik Jiri 114989 author Klein Michael Joséph 41011 author Lambert Paul Henri 25121 author Siegrist Claire-Anne 26816 3390-3400 10.1002/(SICI)1521-4141(199910)29:10<3390::AID-IMMU3390>3.0.CO;2-A 10540351 0014-2980 Early life responses to respiratory syncytial virus (RSV)-F DNA and RSV-F protein immunization were studied in murine models of neonatal immunization. RSV-F DNA induced similar antibody (Ab) responses, antigen-specific IFN-gamma production and cytotoxic T lymphocyte (CTL) responses in 1-week-old and adult BALB / c mice. In contrast, RSV-F protein induced much higher IL-5 responses in early life. Both vaccines elicited Ab and CTL responses in spite of maternal Ab, but with distinctive kinetics. Sequential RSV-F DNA priming / protein boosting primed 1-week-old mice for RSV-F-specific CTL responses, reduced IL-5 production and enhanced Ab responses. In contrast, IL-5 exceeded IFN-gamma responses when young mice were primed with protein and boosted with DNA. Last, when protein and DNA immunization were combined, a single vaccine dose induced early Ab responses, preferential IL-5 responses but strong CTL responses. Sequential or combined DNA / protein immunization thus represent interesting strategies for early life immunization. Animals HN Protein Immunity, Maternally-Acquired Immunoglobulin G/biosynthesis Mice Mice, Inbred BALB C Respiratory Syncytial Virus Infections/prevention & control Respiratory Syncytial Viruses/immunology T-Lymphocytes, Cytotoxic/immunology Th1 Cells/immunology Time Factors Vaccines, Combined/immunology Vaccines, DNA/immunology Viral Envelope Proteins Viral Proteins/immunology Viral Vaccines/immunology Dewey 616.07 Dewey 618 137 Département de pathologie et immunologie 21F 58 Département de pédiatrie, gynécologie et obstétrique 223 false 143 Centre de Vaccinologie et d'Immunologie néonatale 177 European Journal of Immunology 29 10 ba932cee-344f-45e6-853b-bdd7b922ecb8 Article (Published version) Restricted 3390-3400.pdf 196632 application/pdf restrictedAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

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Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice	European Journal of Immunology			1999	527	0

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Klein, Michael Joséph