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Modulation of human cytotrophoblastic leptin secretion by interleukin-1alpha and 17beta-oestradiol and its effect on HCG secretion

Published inMolecular human reproduction, vol. 5, no. 11, p. 1077-1082
Publication date1999
Abstract

To investigate the role of leptin during pregnancy, we assessed leptin production by pure cultured human cytotrophoblastic cells (CTB), its regulation by cytokines and 17beta-oestradiol and its effects on human chorionic gonadotrophin (HCG) secretion. Purified CTB from first trimester placenta were incubated in duplicates in the presence or absence of cytokines or 17beta-oestradiol. Medium was harvested on day 2 and the culture stopped on day 4. Results were corrected for protein content of each individual well and expressed as percent of controls per day (mean +/- SEM). Basal CTB leptin production was 25.2 +/- 2.6 (ng/mg prot). In comparison with controls, leptin production was stimulated to 320 +/- 16% (P < 0.0001) and 195 +/- 3.2% (P < 0.0004) by 3 and 10 ng/ml of interleukin-1alpha respectively. 17beta-oestradiol 10(-6) to 10(-9) mol/l increased basal leptin production 5-9-fold, while 10(-5) mol/l had no such effect. Basal CTB HCG secretion was 5722 +/- 1055 (mIU/mg prot). There was a dose-dependent leptin-induced increase in HCG secretion (P = 0.0039) reaching a 5-fold increase with a leptin concentration of 1 microg/ml (P < 0.006). Gonadotrophin-releasing hormone (GnRH) 8.5 x 10(-8) mol/l significantly increased HCG secretion to 140 +/- 21% of controls (P = 0.031). Cetrorelix (0.1 microg/ml) inhibited leptin-induced HCG secretion (P = 0.0028).

Keywords
  • Cells, Cultured
  • Chorionic Gonadotropin/secretion
  • Estradiol/metabolism
  • Female
  • Gonadotropin-Releasing Hormone/antagonists & inhibitors/metabolism
  • Humans
  • Interleukin-1/metabolism
  • Leptin/metabolism/secretion
  • Pregnancy
  • Recombinant Proteins/metabolism/secretion
  • Trophoblasts/metabolism/secretion
Citation (ISO format)
CHARDONNENS, Didier et al. Modulation of human cytotrophoblastic leptin secretion by interleukin-1alpha and 17beta-oestradiol and its effect on HCG secretion. In: Molecular human reproduction, 1999, vol. 5, n° 11, p. 1077–1082. doi: 10.1093/molehr/5.11.1077
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Article (Published version)
accessLevelPublic
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ISSN of the journal1360-9947
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