Modulation of human cytotrophoblastic leptin secretion by interleukin-1alpha and 17beta-oestradiol and its effect on HCG secretion

Article Article scientifique Article Modulation of human cytotrophoblastic leptin secretion by interleukin-1alpha and 17beta-oestradiol and its effect on HCG secretion eng 1999 author Chardonnens Didier 81049 author Cameo P author Aubert Michel 25310 author Pralong François Pierre 24089 author Islami D author Campana Aldo 22896 author Gaillard Roger 57667 author Bischof Paul 26504 1077-1082 10.1093/molehr/5.11.1077 10541571 1360-9947 To investigate the role of leptin during pregnancy, we assessed leptin production by pure cultured human cytotrophoblastic cells (CTB), its regulation by cytokines and 17beta-oestradiol and its effects on human chorionic gonadotrophin (HCG) secretion. Purified CTB from first trimester placenta were incubated in duplicates in the presence or absence of cytokines or 17beta-oestradiol. Medium was harvested on day 2 and the culture stopped on day 4. Results were corrected for protein content of each individual well and expressed as percent of controls per day (mean +/- SEM). Basal CTB leptin production was 25.2 +/- 2.6 (ng/mg prot). In comparison with controls, leptin production was stimulated to 320 +/- 16% (P < 0.0001) and 195 +/- 3.2% (P < 0.0004) by 3 and 10 ng/ml of interleukin-1alpha respectively. 17beta-oestradiol 10(-6) to 10(-9) mol/l increased basal leptin production 5-9-fold, while 10(-5) mol/l had no such effect. Basal CTB HCG secretion was 5722 +/- 1055 (mIU/mg prot). There was a dose-dependent leptin-induced increase in HCG secretion (P = 0.0039) reaching a 5-fold increase with a leptin concentration of 1 microg/ml (P < 0.006). Gonadotrophin-releasing hormone (GnRH) 8.5 x 10(-8) mol/l significantly increased HCG secretion to 140 +/- 21% of controls (P = 0.031). Cetrorelix (0.1 microg/ml) inhibited leptin-induced HCG secretion (P = 0.0028). Cells, Cultured Chorionic Gonadotropin/secretion Estradiol/metabolism Female Gonadotropin-Releasing Hormone/antagonists & inhibitors/metabolism Humans Interleukin-1/metabolism Leptin/metabolism/secretion Pregnancy Recombinant Proteins/metabolism/secretion Trophoblasts/metabolism/secretion Dewey 616 Dewey 618 56 Département de médecine 221 58 Département de pédiatrie, gynécologie et obstétrique 223 false Molecular human reproduction 5 11 2d7d3b12-687b-46ed-a88f-0e10321b8a6f Article (Published version) Public 1077-1082.pdf 212819 application/pdf openAccess Faculté de médecine / Section de médecine clinique / Département de médecine Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

Title	Published in	Year	Views	Downloads

Obesity and the reproductive axis	Annales d'endocrinologie	2002	422	0
Modulation of human cytotrophoblastic leptin secretion by interleukin-1alpha and 17beta-oestradiol and its effect on HCG secretion	Molecular human reproduction	1999	573	445

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Gaillard, Roger