• Home
  1. Home
  2. Performance of the 47-kilodalton membrane protein versus DNA polymerase i genes for detection of Treponema pallidum by PCR in ulcers
Scientific article
English

Performance of the 47-kilodalton membrane protein versus DNA polymerase i genes for detection of Treponema pallidum by PCR in ulcers

ContributorsGayet-Ageron, Angèleorcid; Laurent, Frédéric; Schrenzel, Jacques; Charton, Béatrice; Jimenez-Getaz, Gisela; Tangomo, Manuela; Ferry, Tristan; Sednaoui, Patrice; Lautenschlager, Stephan; Toutous Trellu, Laurence Marieorcid; Martinez De Tejada Weber, Begona; Cavassini, Matthias; Emonet, Stéphaneorcid; Perneger, Thomas; Salord, Hélène
Published inJournal of clinical microbiology, vol. 53, no. 3, p. 976-980
Publication date2015
Abstract

Treponema pallidum PCR (Tp-PCR) is a direct diagnostic method for primary and secondary syphilis, but there is no recommendation regarding the best choice of target gene. In this study, we sequentially tested 272 specimens from patients with sexually transmitted ulcers using Tp-PCR targeting the tpp47 and then polA genes. The two methods showed similar accuracies and an almost-perfect agreement.

Affiliation entities
Research groups
Citation (ISO format)
GAYET-AGERON, Angèle et al. Performance of the 47-kilodalton membrane protein versus DNA polymerase i genes for detection of Treponema pallidum by PCR in ulcers. In: Journal of clinical microbiology, 2015, vol. 53, n° 3, p. 976–980. doi: 10.1128/JCM.03444-14
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
Journal ISSN0095-1137
715views
0downloads

Technical informations

Creation15/06/2015 16:12:00
First validation15/06/2015 16:12:00
Update13/10/2025 21:20:28
Status update13/01/2025 13:44:31
Last indexation11/12/2025 00:22:11
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack