δ-Conotoxins synthesized using an acid-cleavable solubility tag approach reveal key structural determinants for NaV subtype selectivity

Peigneur, Steve; Paolini-Bertrand, Marianne; Gaertner, Hubert François; Biass, Daniel; Violette, Aude; Stocklin, Reto; Favreau, Philippe; Tytgat, Jan; Hartley, Oliver

doi:10.1074/jbc.M114.610436

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Title		δ-Conotoxins synthesized using an acid-cleavable solubility tag approach reveal key structural determinants for NaV subtype selectivity
Authors		Peigneur, Steve Paolini-Bertrand, Marianne Gaertner, Hubert François Biass, Daniel Violette, Aude Stocklin, Reto Favreau, Philippe Tytgat, Jan Hartley, Oliver show all authors [1 - 9]
Published in		Journal of Biological Chemistry. 2014, vol. 289, no. 51, p. 35341-50
Abstract		Conotoxins are venom peptides from cone snails with multiple disulfide bridges that provide a rigid structural scaffold. Typically acting on ion channels implicated in neurotransmission, conotoxins are of interest both as tools for pharmacological studies and as potential new medicines. δ-Conotoxins act by inhibiting inactivation of voltage-gated sodium channels (Nav). Their pharmacology has not been extensively studied because their highly hydrophobic character makes them difficult targets for chemical synthesis. Here we adopted an acid-cleavable solubility tag strategy that facilitated synthesis, purification, and directed disulfide bridge formation. Using this approach we readily produced three native δ-conotoxins from Conus consors plus two rationally designed hybrid peptides. We observed striking differences in Nav subtype selectivity across this group of compounds, which differ in primary structure at only three positions: 12, 23, and 25. Our results provide new insights into the structure-activity relationships underlying the Nav subtype selectivity of δ-conotoxins. Use of the acid-cleavable solubility tag strategy should facilitate synthesis of other hydrophobic peptides with complex disulfide bridge patterns.
Keywords		Acids/chemistry — Amino Acid Sequence — Animals — Chromatography, High Pressure Liquid — Conotoxins/chemical synthesis/chemistry/pharmacology — Conus Snail/chemistry — Disulfides/chemistry — Dose-Response Relationship, Drug — Female — Ion Channel Gating/drug effects/genetics/physiology — Molecular Sequence Data — Oocytes/drug effects/metabolism/physiology — Peptide Fragments/chemical synthesis/chemistry/pharmacology — Protein Isoforms/genetics/physiology — Solubility — Spectrometry, Mass, Electrospray Ionization — Structure-Activity Relationship — Voltage-Gated Sodium Channels/genetics/physiology — Xenopus laevis
Identifiers		DOI: 10.1074/jbc.M114.610436 PMID: 25352593
Full text		Article (Published version) (2.6 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique
Research group		Hiv (835)
Citation (ISO format)		PEIGNEUR, Steve et al. δ-Conotoxins synthesized using an acid-cleavable solubility tag approach reveal key structural determinants for NaV subtype selectivity. In: Journal of Biological Chemistry, 2014, vol. 289, n° 51, p. 35341-50. doi: 10.1074/jbc.M114.610436 https://archive-ouverte.unige.ch/unige:73894