UNIGE document Scientific Article
previous document  unige:73837  next document
add to browser collection
Title

In Vivo Quantification of 5-HT2A Brain Receptors in Mdr1a KO Rats with 123I-R91150 Single-Photon Emission Computed Tomography

Authors
Moulin-Sallanon, Marcelle
Fender, Pascal
Published in Molecular Imaging. 2015, vol. 14, p. 5-7
Abstract AbstractOur goal was to identify suitable image quantification methods to image 5-hydroxytryptamine2A (5-HT2A) receptors in vivo in Mdr1a knockout (KO) rats (i.e., P-glycoprotein KO) using 123I-R91150 single-photon emission computed tomography (SPECT). The 123I-R91150 binding parameters estimated with different reference tissue models (simplified reference tissue model [SRTM], Logan reference tissue model, and tissue ratio [TR] method) were compared to the estimates obtained with a comprehensive three-tissue/seven-parameter (3T/7k)-based model. The SRTM and Logan reference tissue model estimates of 5-HT2A receptor (5-HT2AR) nondisplaceable binding potential (BPND) correlated well with the absolute receptor density measured with the 3T/7k gold standard (r > .89). Quantification of 5-HT2AR using the Logan reference tissue model required at least 90 minutes of scanning, whereas the SRTM required at least 110 minutes. The TR method estimates were also highly correlated to the 5-HT2AR density (r > .91) and only required a single 20-minute scan between 100 and 120 minutes postinjection. However, a systematic overestimation of the BPND values was observed. The Logan reference tissue method is more convenient than the SRTM for the quantification of 5-HT2AR in Mdr1a KO rats using 123I-R91150 SPECT. The TR method is an interesting and simple alternative, despite its bias, as it still provides a valid index of 5-HT2AR density.
Identifiers
PMID: 26105563
Full text
Article (Published version) (2.4 MB) - public document Free access
Structures
Research groups Groupe Giannakopoulos Panteleimon (psychiatrie générale) (201)
Imagerie de la transmission dopaminergique dans les troubles addictifs (989)
Neuroimagerie moléculaire en psychiatrie (983)
Project FNS: 310030_156829
Citation
(ISO format)
DUMAS, Noe et al. In Vivo Quantification of 5-HT2A Brain Receptors in Mdr1a KO Rats with 123I-R91150 Single-Photon Emission Computed Tomography. In: Molecular Imaging, 2015, vol. 14, p. 5-7. https://archive-ouverte.unige.ch/unige:73837

192 hits

63 downloads

Update

Deposited on : 2015-07-02

Export document
Format :
Citation style :