The regulation of store-dependent Ca2+ influx in HL-60 granulocytes involves GTP-sensitive elements

Jaconi, Marisa; Lew, Daniel Pablo; Monod, Antoinette; Krause, Karl-Heinz

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Title		The regulation of store-dependent Ca2+ influx in HL-60 granulocytes involves GTP-sensitive elements
Authors		Jaconi, Marisa Lew, Daniel Pablo Monod, Antoinette Krause, Karl-Heinz
Published in		Journal of Biological Chemistry. 1993, vol. 268, no. 35, p. 26075-26078
Abstract		In granulocytes, emptying of intracellular Ca2+ stores activates Ca2+ influx across the plasma membrane. To study the putative role of GTP-binding proteins in this process, we have introduced non-hydrolyzable guanosine phosphate analogues into the cytosol of non-permeabilized HL-60 granulocytes using an endocytosis-hypoosmotic shock procedure. At the cytosolic concentrations obtained (100-500 microM), neither guanosine 5'-3-O-(thio)triphosphate (GTP gamma S) nor guanosine 5'-3-O-(thio)diphosphate (GDP beta S) affected basal [Ca2+]i. Ca2+ release in response to the receptor agonist fMet-Leu-Phe, the Ca(2+)-ATPase inhibitor thapsigargin, or the Ca2+ ionophore ionomycin was also unaffected by GTP gamma S or GDP beta S. In contrast, the activation of the Ca2+ influx pathway by fMet-Leu-Phe or by thapsigargin was blocked by GTP gamma S but not by GDP beta S. The GTP gamma S effect was mimicked by NaF. The GTP gamma S and NaF effects were independent of protein kinase C activation and actin polymerization. Our results demonstrate that a GTP-sensitive element is involved in the signaling between intracellular Ca2+ stores and plasma membrane Ca2+ channels. The identical effects of GTP gamma S and NaF suggest that the GTP-sensitive element is a heterotrimeric G-protein.
Keywords		Biological Transport — Calcium/ metabolism — Cell Line — Granulocytes/drug effects/ metabolism — Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology — Guanosine Diphosphate/analogs & derivatives/pharmacology — Guanosine Triphosphate/ metabolism — N-Formylmethionine Leucyl-Phenylalanine/pharmacology — Sodium Fluoride/pharmacology — Terpenes/pharmacology — Tetradecanoylphorbol Acetate/pharmacology — Thapsigargin — Thionucleotides/pharmacology
Identifiers		PMID: 8253721
Full text		Article - Limited access to UNIGE Other version: http://www.jbc.org/content/268/35/26075.full.pdf
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Citation (ISO format)		JACONI, Marisa et al. The regulation of store-dependent Ca2+ influx in HL-60 granulocytes involves GTP-sensitive elements. In: Journal of Biological Chemistry, 1993, vol. 268, n° 35, p. 26075-26078. https://archive-ouverte.unige.ch/unige:7351