Scientific article

The regulation of store-dependent Ca2+ influx in HL-60 granulocytes involves GTP-sensitive elements

Published inThe Journal of biological chemistry, vol. 268, no. 35, p. 26075-26078
Publication date1993

In granulocytes, emptying of intracellular Ca2+ stores activates Ca2+ influx across the plasma membrane. To study the putative role of GTP-binding proteins in this process, we have introduced non-hydrolyzable guanosine phosphate analogues into the cytosol of non-permeabilized HL-60 granulocytes using an endocytosis-hypoosmotic shock procedure. At the cytosolic concentrations obtained (100-500 microM), neither guanosine 5'-3-O-(thio)triphosphate (GTP gamma S) nor guanosine 5'-3-O-(thio)diphosphate (GDP beta S) affected basal [Ca2+]i. Ca2+ release in response to the receptor agonist fMet-Leu-Phe, the Ca(2+)-ATPase inhibitor thapsigargin, or the Ca2+ ionophore ionomycin was also unaffected by GTP gamma S or GDP beta S. In contrast, the activation of the Ca2+ influx pathway by fMet-Leu-Phe or by thapsigargin was blocked by GTP gamma S but not by GDP beta S. The GTP gamma S effect was mimicked by NaF. The GTP gamma S and NaF effects were independent of protein kinase C activation and actin polymerization. Our results demonstrate that a GTP-sensitive element is involved in the signaling between intracellular Ca2+ stores and plasma membrane Ca2+ channels. The identical effects of GTP gamma S and NaF suggest that the GTP-sensitive element is a heterotrimeric G-protein.

  • Biological Transport
  • Calcium/ metabolism
  • Cell Line
  • Granulocytes/drug effects/ metabolism
  • Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
  • Guanosine Diphosphate/analogs & derivatives/pharmacology
  • Guanosine Triphosphate/ metabolism
  • N-Formylmethionine Leucyl-Phenylalanine/pharmacology
  • Sodium Fluoride/pharmacology
  • Terpenes/pharmacology
  • Tetradecanoylphorbol Acetate/pharmacology
  • Thapsigargin
  • Thionucleotides/pharmacology
Citation (ISO format)
JACONI, Marisa et al. The regulation of store-dependent Ca2+ influx in HL-60 granulocytes involves GTP-sensitive elements. In: The Journal of biological chemistry, 1993, vol. 268, n° 35, p. 26075–26078.
Updates (1)
ISSN of the journal0021-9258

Technical informations

Creation06/21/2010 10:24:55 AM
First validation06/21/2010 10:24:55 AM
Update time03/14/2023 3:44:15 PM
Status update03/14/2023 3:44:15 PM
Last indexation10/18/2023 9:24:43 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack