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Cointegrate formation by Tn5, but not transposition, is dependent on recA

Galas, David J.
Chandler, Michael
Published in Proceedings of the National Academy of Sciences of the United States of America. 1982, vol. 79, no. 15, p. 4530-4534
Abstract We have studied the effect of the recA-dependent homologous recombination system of Escherichia coli on both Tn5-mediated cointegrate formation and Tn5 transposition. We demonstrate here that, whereas transposition of Tn5 is independent of the recA gene product (as has been shown by other workers), Tn5-mediated cointegrate formation is strongly dependent on recA. The structures of both the simple transposition products and the cointegrates formed in a recA- background seem to be the same as those produced in a recA+ background. These results provide strong evidence that Tn5 does not transpose via an obligate cointegrate intermediate and suggest that the recA effect on cointegrate formation is exerted during the process of transposition.
Keywords Bacterial Proteins/ physiologyDNA Transposable ElementsEscherichia coli/geneticsPlasmidsRec A RecombinasesRecombination, Genetic
PMID: 6289304
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HIRSCHEL, Bernard, GALAS, David J., CHANDLER, Michael. Cointegrate formation by Tn5, but not transposition, is dependent on recA. In: Proceedings of the National Academy of Sciences, 1982, vol. 79, n° 15, p. 4530-4534. doi: 10.1073/pnas.79.15.4530 https://archive-ouverte.unige.ch/unige:7333

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Deposited on : 2010-06-21

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