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Scientific article
Open access
English

Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing

Published inBiosensors, vol. 5, no. 2, p. 319-336
Publication date2015
Abstract

An alternative current (AC) dielectrophoretic lab-on-chip setup was evaluated as a rapid tool of capture and assembly of microalga Chlamydomonas reinhardtii in two-dimensional (2D) close-packed arrays. An electric field of 100 V·cm−1, 100 Hz applied for 30 min was found optimal to collect and assemble the algae into single-layer structures of closely packed cells without inducing cellular oxidative stress. Combined with oxidative stress specific staining and fluorescence microscopy detection, the capability of using the 2D whole-cell assembly on-chip to follow the reactive oxygen species (ROS) production and oxidative stress during short-term exposure to several environmental contaminants, including mercury, methylmercury, copper, copper oxide nanoparticles (CuO-NPs), and diuron was explored. The results showed significant increase of the cellular ROS when C. reinhardtii was exposed to high concentrations of methylmercury, CuO-NPs, and 10−5 M Cu. Overall, this study demonstrates the potential of combining AC-dielectrophoretically assembled two-dimensional algal structures with cell metabolic analysis using fluorescence staining, as a rapid analytical tool for probing the effect of contaminants in highly impacted environment.

Keywords
  • Whole cell assembly
  • Dielectrophoresis
  • Fluorescence
  • Contamination
  • Reactive oxygen species
  • Chlamydomonas reinhardtii
Funding
  • Swiss National Science Foundation - 200021-152897
  • Autre - Foundation Marc Birkigt, Geneva
Citation (ISO format)
SUSCILLON, Coralie, VELEV, Orlin, SLAVEYKOVA, Vera. Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing. In: Biosensors, 2015, vol. 5, n° 2, p. 319–336. doi: 10.3390/bios5020319
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Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal2079-6374
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Creation06/16/2015 3:04:00 PM
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