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Title

Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing

Authors
Velev, Orlin
Published in Biosensors. 2015, vol. 5, no. 2, p. 319-336
Abstract An alternative current (AC) dielectrophoretic lab-on-chip setup was evaluated as a rapid tool of capture and assembly of microalga Chlamydomonas reinhardtii in two-dimensional (2D) close-packed arrays. An electric field of 100 V·cm−1, 100 Hz applied for 30 min was found optimal to collect and assemble the algae into single-layer structures of closely packed cells without inducing cellular oxidative stress. Combined with oxidative stress specific staining and fluorescence microscopy detection, the capability of using the 2D whole-cell assembly on-chip to follow the reactive oxygen species (ROS) production and oxidative stress during short-term exposure to several environmental contaminants, including mercury, methylmercury, copper, copper oxide nanoparticles (CuO-NPs), and diuron was explored. The results showed significant increase of the cellular ROS when C. reinhardtii was exposed to high concentrations of methylmercury, CuO-NPs, and 10−5 M Cu. Overall, this study demonstrates the potential of combining AC-dielectrophoretically assembled two-dimensional algal structures with cell metabolic analysis using fluorescence staining, as a rapid analytical tool for probing the effect of contaminants in highly impacted environment.
Keywords Whole cell assemblyDielectrophoresisFluorescenceContaminationReactive oxygen speciesChlamydomonas reinhardtii
Identifiers
Full text
Structures
Research groups Environmental Biogeochemistry and Ecotoxicology
ISE Eau
Projects FNS: 200021-152897
Foundation Marc Birkigt, Geneva
Citation
(ISO format)
SUSCILLON, Coralie, VELEV, Orlin, SLAVEYKOVA, Vera. Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing. In: Biosensors, 2015, vol. 5, n° 2, p. 319-336. https://archive-ouverte.unige.ch/unige:73237

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Deposited on : 2015-06-17

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