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Title

Assembly of PNA-Tagged Small Molecules, Peptides, and Carbohydrates onto DNA Templates: Programming the Combinatorial Pairing and Inter-ligand Distance

Authors
Gorska, Katarzyna
Ciobanu, Mihai
Published in Nielsen, Peter E. & Appella, Daniel H. Peptide Nucleic Acids : Methods and Protocols. Totowa, NJ: Humana Press. 2014, p. 95-110
Collection Methods in Molecular Biology; 1050
Abstract The biochemical stability and desirable hybridization properties of peptide nucleic acids (PNA) coupled to the robustness of the peptidic chemistry involved in their oligomerization make them an attractive nucleic acid tag to encode molecules and program their assembly into higher order oligomers. The ability to program the dimerization of ligands with controlled distance between the ligands has important applications in emulating multimeric interactions. Additionally, the ability to program different permutations of ligand assemblies in a combinatorial fashion provides access to a broad diversity and offers a rapid screening method for fragment based approaches to drug discovery. Herein, we describe protocols to covalently link diverse carbohydrates, peptides, or small molecules to PNA and combinatorially assemble them in solution onto libraries of DNA templates or onto DNA microarrays using a commercial platform without recourse to specialized equipment or heavy upfront investment.
Keywords Peptide nucleic acid (PNA)MicroarrayEncoded librariesCombinatorial self-assemblyScreeningCarbohydratesMultivalent interactions
Identifiers
ISBN: 978-1-62703-552-1
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(ISO format)
WINSSINGER, Nicolas et al. Assembly of PNA-Tagged Small Molecules, Peptides, and Carbohydrates onto DNA Templates: Programming the Combinatorial Pairing and Inter-ligand Distance. In: Nielsen, Peter E. & Appella, Daniel H. (Ed.). Peptide Nucleic Acids : Methods and Protocols. Totowa, NJ : Humana Press, 2014. p. 95-110. (Methods in Molecular Biology; 1050) https://archive-ouverte.unige.ch/unige:72888

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Deposited on : 2015-06-01

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