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A low-fibronectin-binding mutant of Staphylococcus aureus 879R4S has Tn918 inserted into its single fnb gene

Greene, C.
Proctor, R. A.
McDevitt, D.
Foster, T. J.
Published in Microbiology. 1996, vol. 142, no. Pt 8, p. 2153-2160
Abstract A low-fibronectin-binding mutant of Staphylococcus aureus strain 879R4SSp generated by transposon Tn918 mutagenesis is attenuated in a rat endocarditis model (J.M. Kuypers & R.A. Procter, 1989, Infect Immun 57, 2306-2312). PCR and Southern hybridization analysis with primers and probes, respectively, for the fnbA and fnbB genes of strains 8325-4 showed that strain 879R4SSp possesses a single fnb gene which is homologous to fnbA. This was confirmed by sequencing 41 bp of 5' non-coding and 237 bp of 5' coding DNA, which showed 97% base identity to fnbA. Southern hybridization and sequencing showed that Tn918 was inserted 41 bp 5' to fnbA in the mutant 879R4SSp/1536, between the promoter and initiation codon. Reduced adherence of the mutant to surface-bound fibronectin correlated with lower expression of a 180 kDa wall-associated fibronectin-binding protein.
Keywords Adhesins, BacterialAnimalsBacterial AdhesionBacterial Outer Membrane Proteins/biosynthesis/ geneticsBacterial ProteinsBase SequenceBlotting, SouthernCarrier ProteinsDNA PrimersDNA Transposable ElementsEndocarditis/microbiologyEscherichia coliFibronectins/ metabolismGenotypeMolecular Sequence DataMutagenesis, InsertionalOligonucleotide ProbesPolymerase Chain ReactionRatsRestriction MappingStaphylococcal Infections/microbiologyStaphylococcus aureus/genetics/ physiology
PMID: 8760929
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GREENE, C. et al. A low-fibronectin-binding mutant of Staphylococcus aureus 879R4S has Tn918 inserted into its single fnb gene. In: Microbiology, 1996, vol. 142, n° Pt 8, p. 2153-2160. doi: 10.1099/13500872-142-8-2153 https://archive-ouverte.unige.ch/unige:7281

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