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A low-fibronectin-binding mutant of Staphylococcus aureus 879R4S has Tn918 inserted into its single fnb gene

Publié dansMicrobiology, vol. 142, no. Pt 8, p. 2153-2160
Date de publication1996
Résumé

A low-fibronectin-binding mutant of Staphylococcus aureus strain 879R4SSp generated by transposon Tn918 mutagenesis is attenuated in a rat endocarditis model (J.M. Kuypers & R.A. Procter, 1989, Infect Immun 57, 2306-2312). PCR and Southern hybridization analysis with primers and probes, respectively, for the fnbA and fnbB genes of strains 8325-4 showed that strain 879R4SSp possesses a single fnb gene which is homologous to fnbA. This was confirmed by sequencing 41 bp of 5' non-coding and 237 bp of 5' coding DNA, which showed 97% base identity to fnbA. Southern hybridization and sequencing showed that Tn918 was inserted 41 bp 5' to fnbA in the mutant 879R4SSp/1536, between the promoter and initiation codon. Reduced adherence of the mutant to surface-bound fibronectin correlated with lower expression of a 180 kDa wall-associated fibronectin-binding protein.

Mots-clés
  • Adhesins, Bacterial
  • Animals
  • Bacterial Adhesion
  • Bacterial Outer Membrane Proteins/biosynthesis/ genetics
  • Bacterial Proteins
  • Base Sequence
  • Blotting, Southern
  • Carrier Proteins
  • DNA Primers
  • DNA Transposable Elements
  • Endocarditis/microbiology
  • Escherichia coli
  • Fibronectins/ metabolism
  • Genotype
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Oligonucleotide Probes
  • Polymerase Chain Reaction
  • Rats
  • Restriction Mapping
  • Staphylococcal Infections/microbiology
  • Staphylococcus aureus/genetics/ physiology
Citation (format ISO)
GREENE, C. et al. A low-fibronectin-binding mutant of Staphylococcus aureus 879R4S has Tn918 inserted into its single fnb gene. In: Microbiology, 1996, vol. 142, n° Pt 8, p. 2153–2160. doi: 10.1099/13500872-142-8-2153
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Identifiants
ISSN du journal1350-0872
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Création21.06.2010 10:24:29
Première validation21.06.2010 10:24:29
Heure de mise à jour14.03.2023 15:43:53
Changement de statut14.03.2023 15:43:53
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