en
Scientific article
English

Comparison of fluorescence and resonance light scattering for highly sensitive microarray detection of bacterial pathogens

Published inJournal of microbiological methods, vol. 55, no. 3, p. 755-762
Publication date2003
Abstract

Microarrays have emerged as potential tools for bacterial detection and identification. Given their high parallelism, they might represent a breakthrough in current diagnostic methods, provided they can be coupled to simplified labeling protocols and detected with adequate sensitivities. We describe here a technique to directly label total bacterial RNA, thus avoiding the multiple steps and possible biases associated with enzymatic amplification (e.g. PCR). We have then compared the performances of one white-light source and two laser-based fluorescence scanners for detection reliability and sensitivity. Our study reveals that nanoparticle-labeled bacterial RNA generates reproducible resonance light scattering signals that are at least 50 times more intense than state-of-the-art confocal-based fluorescence signals.

Keywords
  • Light
  • Microscopy, Fluorescence
  • Oligonucleotide Array Sequence Analysis/ methods
  • RNA, Bacterial/chemistry/genetics
  • Reproducibility of Results
  • Scattering, Radiation
  • Sensitivity and Specificity
  • Staphylococcus aureus/ genetics/isolation & purification
Citation (ISO format)
FRANCOIS, Patrice et al. Comparison of fluorescence and resonance light scattering for highly sensitive microarray detection of bacterial pathogens. In: Journal of microbiological methods, 2003, vol. 55, n° 3, p. 755–762. doi: 10.1016/j.mimet.2003.08.008
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ISSN of the journal0167-7012
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