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Title

Comparison of fluorescence and resonance light scattering for highly sensitive microarray detection of bacterial pathogens

Authors
Bento, Manuela
Published in Journal of Microbiological Methods. 2003, vol. 55, no. 3, p. 755-762
Abstract Microarrays have emerged as potential tools for bacterial detection and identification. Given their high parallelism, they might represent a breakthrough in current diagnostic methods, provided they can be coupled to simplified labeling protocols and detected with adequate sensitivities. We describe here a technique to directly label total bacterial RNA, thus avoiding the multiple steps and possible biases associated with enzymatic amplification (e.g. PCR). We have then compared the performances of one white-light source and two laser-based fluorescence scanners for detection reliability and sensitivity. Our study reveals that nanoparticle-labeled bacterial RNA generates reproducible resonance light scattering signals that are at least 50 times more intense than state-of-the-art confocal-based fluorescence signals.
Keywords LightMicroscopy, FluorescenceOligonucleotide Array Sequence Analysis/ methodsRNA, Bacterial/chemistry/geneticsReproducibility of ResultsScattering, RadiationSensitivity and SpecificityStaphylococcus aureus/ genetics/isolation & purification
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PMID: 14607418
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FRANCOIS, Patrice et al. Comparison of fluorescence and resonance light scattering for highly sensitive microarray detection of bacterial pathogens. In: Journal of Microbiological Methods, 2003, vol. 55, n° 3, p. 755-762. https://archive-ouverte.unige.ch/unige:7225

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Deposited on : 2010-06-21

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