

Other version: http://www.intmedpress.com/journals/avt/abstract.cfm?id=1197&pid=88
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Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo |
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Published in | Antiviral therapy. 2003, vol. 8, no. 2, p. 97-104 | |
Abstract | The significance of distinct classes of HIV-1 nucleic acids as correlates of recent HIV-1 replication was assessed in peripheral blood mononuclear cells (PBMC) obtained from 14 patients during 2 weeks of structured interruption of antiretroviral therapy (STI) and 2 weeks of resuming therapy. Levels of HIV RNA in plasma (HIV-RNAplasma) and of unspliced cell-associated HIV-1 RNA (HIV-UsRNAPBMC) were significantly increased as a result of STI, whereas no significant shifts in the levels of 2-LTR episomal HIV-1 DNA (2-LTR circles) and total late HIV-1 reverse transcripts (late-DNA) were observed. Thus, limited viral replication had occurred, which had no effect on the pool size of infected cells in the periphery. Levels of 2-LTR circles did not reflect rapid changes in HIV-1 replication. In contrast, expression of HIV-UsRNAPBMC increased during STI and consequently provides a more sensitive, albeit not absolute cellular marker of ongoing HIV-1 replication. | |
Keywords | Anti-HIV Agents/administration & dosage/ therapeutic use — Biological Markers/blood — CD4-Positive T-Lymphocytes/drug effects/virology — Cell Extracts/genetics — Cells, Cultured — DNA, Viral/ blood — Drug Therapy, Combination — HIV Infections/blood/ drug therapy — HIV Long Terminal Repeat — HIV-1/chemistry/ genetics — Humans — Leukocytes, Mononuclear/ virology — Longitudinal Studies — Prospective Studies — RNA, Viral/ blood — Virus Replication | |
Identifiers | PMID: 12741621 | |
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![]() ![]() Other version: http://www.intmedpress.com/journals/avt/abstract.cfm?id=1197&pid=88 |
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Citation (ISO format) | FISCHER, Marek et al. Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo. In: Antiviral therapy, 2003, vol. 8, n° 2, p. 97-104. https://archive-ouverte.unige.ch/unige:7215 |