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Scientific article
English

Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo

Published inAntiviral therapy, vol. 8, no. 2, p. 97-104
Publication date2003
Abstract

The significance of distinct classes of HIV-1 nucleic acids as correlates of recent HIV-1 replication was assessed in peripheral blood mononuclear cells (PBMC) obtained from 14 patients during 2 weeks of structured interruption of antiretroviral therapy (STI) and 2 weeks of resuming therapy. Levels of HIV RNA in plasma (HIV-RNAplasma) and of unspliced cell-associated HIV-1 RNA (HIV-UsRNAPBMC) were significantly increased as a result of STI, whereas no significant shifts in the levels of 2-LTR episomal HIV-1 DNA (2-LTR circles) and total late HIV-1 reverse transcripts (late-DNA) were observed. Thus, limited viral replication had occurred, which had no effect on the pool size of infected cells in the periphery. Levels of 2-LTR circles did not reflect rapid changes in HIV-1 replication. In contrast, expression of HIV-UsRNAPBMC increased during STI and consequently provides a more sensitive, albeit not absolute cellular marker of ongoing HIV-1 replication.

Keywords
  • Anti-HIV Agents/administration & dosage/ therapeutic use
  • Biological Markers/blood
  • CD4-Positive T-Lymphocytes/drug effects/virology
  • Cell Extracts/genetics
  • Cells, Cultured
  • DNA, Viral/ blood
  • Drug Therapy, Combination
  • HIV Infections/blood/ drug therapy
  • HIV Long Terminal Repeat
  • HIV-1/chemistry/ genetics
  • Humans
  • Leukocytes, Mononuclear/ virology
  • Longitudinal Studies
  • Prospective Studies
  • RNA, Viral/ blood
  • Virus Replication
Citation (ISO format)
FISCHER, Marek et al. Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo. In: Antiviral therapy, 2003, vol. 8, n° 2, p. 97–104.
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ISSN of the journal1359-6535
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