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Title

Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo

Authors
Fischer, Marek
Trkola, Alexandra
Joos, Beda
Hafner, Roland
Joller, Helen
Muesing, M. A.
Kaufman, D. R.
Berli, Esther
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Published in Antiviral Therapy. 2003, vol. 8, no. 2, p. 97-104
Abstract The significance of distinct classes of HIV-1 nucleic acids as correlates of recent HIV-1 replication was assessed in peripheral blood mononuclear cells (PBMC) obtained from 14 patients during 2 weeks of structured interruption of antiretroviral therapy (STI) and 2 weeks of resuming therapy. Levels of HIV RNA in plasma (HIV-RNAplasma) and of unspliced cell-associated HIV-1 RNA (HIV-UsRNAPBMC) were significantly increased as a result of STI, whereas no significant shifts in the levels of 2-LTR episomal HIV-1 DNA (2-LTR circles) and total late HIV-1 reverse transcripts (late-DNA) were observed. Thus, limited viral replication had occurred, which had no effect on the pool size of infected cells in the periphery. Levels of 2-LTR circles did not reflect rapid changes in HIV-1 replication. In contrast, expression of HIV-UsRNAPBMC increased during STI and consequently provides a more sensitive, albeit not absolute cellular marker of ongoing HIV-1 replication.
Keywords Anti-HIV Agents/administration & dosage/ therapeutic useBiological Markers/bloodCD4-Positive T-Lymphocytes/drug effects/virologyCell Extracts/geneticsCells, CulturedDNA, Viral/ bloodDrug Therapy, CombinationHIV Infections/blood/ drug therapyHIV Long Terminal RepeatHIV-1/chemistry/ geneticsHumansLeukocytes, Mononuclear/ virologyLongitudinal StudiesProspective StudiesRNA, Viral/ bloodVirus Replication
Identifiers
PMID: 12741621
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FISCHER, Marek et al. Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo. In: Antiviral Therapy, 2003, vol. 8, n° 2, p. 97-104. https://archive-ouverte.unige.ch/unige:7215

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Deposited on : 2010-06-21

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