Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo

Fischer, Marek; Trkola, Alexandra; Joos, Beda; Hafner, Roland; Joller, Helen; Muesing, M. A.; Kaufman, D. R.; Berli, Esther; Hirschel, Bernard; Weber, Rainer; Gunthard, Huldrych

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Title		Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo
Authors		Fischer, Marek Trkola, Alexandra Joos, Beda Hafner, Roland Joller, Helen Muesing, M. A. Kaufman, D. R. Berli, Esther Hirschel, Bernard Weber, Rainer Gunthard, Huldrych show all authors [1 - 11]
Published in		Antiviral Therapy. 2003, vol. 8, no. 2, p. 97-104
Abstract		The significance of distinct classes of HIV-1 nucleic acids as correlates of recent HIV-1 replication was assessed in peripheral blood mononuclear cells (PBMC) obtained from 14 patients during 2 weeks of structured interruption of antiretroviral therapy (STI) and 2 weeks of resuming therapy. Levels of HIV RNA in plasma (HIV-RNAplasma) and of unspliced cell-associated HIV-1 RNA (HIV-UsRNAPBMC) were significantly increased as a result of STI, whereas no significant shifts in the levels of 2-LTR episomal HIV-1 DNA (2-LTR circles) and total late HIV-1 reverse transcripts (late-DNA) were observed. Thus, limited viral replication had occurred, which had no effect on the pool size of infected cells in the periphery. Levels of 2-LTR circles did not reflect rapid changes in HIV-1 replication. In contrast, expression of HIV-UsRNAPBMC increased during STI and consequently provides a more sensitive, albeit not absolute cellular marker of ongoing HIV-1 replication.
Keywords		Anti-HIV Agents/administration & dosage/ therapeutic use — Biological Markers/blood — CD4-Positive T-Lymphocytes/drug effects/virology — Cell Extracts/genetics — Cells, Cultured — DNA, Viral/ blood — Drug Therapy, Combination — HIV Infections/blood/ drug therapy — HIV Long Terminal Repeat — HIV-1/chemistry/ genetics — Humans — Leukocytes, Mononuclear/ virology — Longitudinal Studies — Prospective Studies — RNA, Viral/ blood — Virus Replication
Identifiers		PMID: 12741621
Full text		Article - Limited access to UNIGE Other version: http://www.intmedpress.com/journals/avt/abstract.cfm?id=1197&pid=88
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Citation (ISO format)		FISCHER, Marek et al. Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivo. In: Antiviral Therapy, 2003, vol. 8, n° 2, p. 97-104. https://archive-ouverte.unige.ch/unige:7215