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Scientific article
English

Attenuated and nonproductive viral transcription in the lymphatic tissue of HIV-1-infected patients receiving potent antiretroviral therapy

Contributors

Fischer, Marek; Joos, Beda; Wong, J. K.; Ott, Peter; Opravil, Milos; Hirschel, Bernard; Weber, Rainer; Gunthard, Huldrych

Published in

The Journal of infectious diseases, vol. 189, no. 2, p. 273-285

Publication date

2004

Abstract

Human immunodeficiency virus type 1 (HIV-1) RNA that persists in the lymphoid tissue of patients despite treatment with highly active antiretroviral therapy (HAART) may represent extracellular virions or intracellular RNAs residing within HIV-infected cells. To further characterize residual viral transcription, tonsil biopsy specimens from patients receiving long-term HAART, untreated patients, and patients undergoing 2 weeks of structured treatment interruption were analyzed by polymerase chain reaction quantification of virion-encapsidated RNA, intracellular unspliced HIV RNA (HIV UsRNA), multiply spliced HIV RNA encoding tat and rev (HIV MsRNA), and HIV DNA. Tonsil biopsy specimens from viremic patients harbored high amounts of virions, which primarily stemmed from local production, as indicated by a strong correlation of extracellular tonsillar RNA with intracellular HIV-1 nucleic acid levels but not with plasma viremia, and as shown by phylogenetic analysis of clonal env sequences from lymphoid tissue and plasma. In patients receiving HAART, intracellular HIV UsRNA persisted at significantly decreased levels, whereas HIV MsRNA and lymphoid virion levels were depleted. Thus, residual lymphoid HIV-1 RNA in patients receiving HAART indicates attenuated viral transcription in HIV-1-infected cells that lack virion production.

Keywords

  • Acquired Immunodeficiency Syndrome/ drug therapy/virology
  • Antiretroviral Therapy, Highly Active
  • HIV-1/classification/ genetics
  • Humans
  • Lymphoid Tissue/ virology
  • Phylogeny
  • RNA, Viral/blood
  • Transcription, Genetic
  • Viremia/virology

UNIGE affiliation entities

Not a UNIGE publication

Citation (ISO format)

FISCHER, Marek et al. Attenuated and nonproductive viral transcription in the lymphatic tissue of HIV-1-infected patients receiving potent antiretroviral therapy. In: The Journal of infectious diseases, 2004, vol. 189, n° 2, p. 273–285. doi: 10.1086/380797

Main files (1)

Article

Identifiers

Journal ISSN

0022-1899
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Technical informations

Creation

21/06/2010 10:24:02

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21/06/2010 10:24:02

Update

14/03/2023 15:43:30

Status update

14/03/2023 15:43:30

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