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Article scientifique
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Cellular viral rebound after cessation of potent antiretroviral therapy predicted by levels of multiply spliced HIV-1 RNA encoding nef

Publié dansThe Journal of infectious diseases, vol. 190, no. 11, p. 1979-1988
Date de publication2004
Résumé

To characterize newly arising replication of human immunodeficiency virus (HIV) type 1 in vivo at the cellular level, distinct viral RNA species in peripheral blood mononuclear cells (PBMCs) from HIV-1-infected patients were monitored during 2 weeks of structured treatment interruption (STI). HIV-1 RNA encoding tat/rev and PBMC-associated virions were almost completely depleted during antiretroviral therapy and emerged simultaneously after 2 weeks of STI, thus specifically reflecting productive viral infection at the cellular level. The magnitude of these correlates of reappearing cellular viral replication was predicted by during-therapy levels of nef transcripts in PBMCs. Significant rebound of plasma viremia, representing the progeny of a broader range of anatomical compartments, preceded and predicted productive infection in PBMCs. Thus, cellular viral rebound in PBMCs likely was primed before STI by the expression of nef in HIV-1-infected PBMCs that lacked virion production and was subsequently triggered by the plasma viremia that preceded the recurrence of productively infected PBMCs.

Mots-clés
  • Anti-HIV Agents/ therapeutic use
  • Cells, Cultured
  • Drug Therapy, Combination
  • Gene Products, nef/analysis/ biosynthesis
  • Gene Products, rev/metabolism
  • Gene Products, tat/metabolism
  • HIV Infections/blood/ drug therapy/virology
  • HIV-1/genetics/ isolation & purification
  • Humans
  • Leukocytes, Mononuclear/ virology
  • Neutrophils/virology
  • RNA Splicing
  • RNA, Viral/analysis/ biosynthesis
  • Recurrence
  • Virus Replication
  • Nef Gene Products, Human Immunodeficiency Virus
  • Rev Gene Products, Human Immunodeficiency Virus
  • Tat Gene Products, Human Immunodeficiency Virus
Citation (format ISO)
FISCHER, Marek et al. Cellular viral rebound after cessation of potent antiretroviral therapy predicted by levels of multiply spliced HIV-1 RNA encoding nef. In: The Journal of infectious diseases, 2004, vol. 190, n° 11, p. 1979–1988. doi: 10.1086/425983
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Identifiants
ISSN du journal0022-1899
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Création21.06.2010 10:24:01
Première validation21.06.2010 10:24:01
Heure de mise à jour14.03.2023 15:43:30
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