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Scientific article
English

Cloning and analysis of the K1 capsule biosynthesis genes of Escherichia coli: lack of homology with Neisseria meningitidis group B DNA sequences

Contributors

Echarti, C.; Hirschel, Bernard; Boulnois, G. J.; Varley, J. M.; Waldvogel, Francis; Timmis, K. N.

Published in

Infection and immunity, vol. 41, no. 1, p. 54-60

Publication date

1983

Abstract

Genes coding for production of the K1 polysaccharide capsule of Escherichia coli have been cloned. Complementation, insertion, and deletion analyses were used to localize the K1 genes and demonstrated that a minimum of 9 kilobases of DNA split into at least two gene blocks is involved in synthesis and assembly of the capsule. One of the gene blocks is responsible for biosynthesis of the polysaccharide, and the other is responsible for extracellular appearance of capsular material. Using cloned K1 genes as probes in Southern blot experiments, we detected homology to DNA from strains of E. coli capsular types K92, K7, and K100. In contrast, no homology was apparent between K1 genes and DNA from meningococcus group B, although the K1 and group B capsules are chemically and immunologically identical.

Keywords

  • Chromosome Mapping
  • Chromosomes, Bacterial
  • Cloning, Molecular
  • DNA, Bacterial
  • Escherichia coli/ genetics
  • Genes
  • Genes, Bacterial
  • Neisseria meningitidis/ genetics
  • Nucleic Acid Hybridization
  • Plasmids
  • Polysaccharides, Bacterial/ genetics

UNIGE affiliation entities

Citation (ISO format)

ECHARTI, C. et al. Cloning and analysis of the K1 capsule biosynthesis genes of Escherichia coli: lack of homology with Neisseria meningitidis group B DNA sequences. In: Infection and immunity, 1983, vol. 41, n° 1, p. 54–60.

Main files (1)

Article

Identifiers

Additional URL for this publication

http://iai.asm.org/cgi/reprint/41/1/54.pdf

Journal ISSN

0019-9567
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