en
Scientific article
English

Simultaneous detection of parainfluenza viruses 1 and 3 by real-time reverse transcription-polymerase chain reaction

Published inJournal of virological methods, vol. 156, no. 1-2, p. 166-168
Publication date2009
Abstract

Human parainfluenza virus (HPIV) types 1 and 3 are major viral pathogens responsible for upper and lower respiratory tract infections. The diagnosis of these two species is achieved generally by specific reverse transcription-polymerase chain (RT-PCR) reaction methods. In this study, a real-time RT-PCR was developed using a common pair of primers-probe (HPIV-1+3) for the simultaneous detection of both HPIV-1 and HPIV-3 genomes. Results obtained in a 10-fold dilution series assay demonstrate a high sensitivity of the assay with a lowest detection limit of approximately one plasmid copy for both HPIV-1 and HPIV-3. A comparison of HPIV-1 and HPIV-3 clinical sample detection between specific HPIV-1/HPIV-3 pairs of primers-probes and the HPIV-1+3 combination clearly shows that the latter is significantly more sensitive (gain of about five threshold cycles) than the former for HPIV-3 detection, while equivalent values are observed for HPIV-1. The HPIV-1+3 combination constitutes a more rapid, more sensitive, and less expensive alternative than classical or multiplex real-time RT-PCR assays usually used in clinical laboratories.

Keywords
  • DNA Primers
  • Humans
  • Parainfluenza Virus 1, Human/genetics/ isolation & purification
  • Parainfluenza Virus 3, Human/genetics/ isolation & purification
  • RNA, Viral/analysis
  • Respirovirus Infections/ diagnosis
  • Reverse Transcriptase Polymerase Chain Reaction/ methods
  • Sensitivity and Specificity
Citation (ISO format)
CORDEY, Samuel et al. Simultaneous detection of parainfluenza viruses 1 and 3 by real-time reverse transcription-polymerase chain reaction. In: Journal of virological methods, 2009, vol. 156, n° 1-2, p. 166–168. doi: 10.1016/j.jviromet.2008.11.006
Main files (1)
Article
accessLevelRestricted
Identifiers
ISSN of the journal0166-0934
555views
0downloads

Technical informations

Creation06/21/2010 10:23:36 AM
First validation06/21/2010 10:23:36 AM
Update time03/14/2023 3:43:07 PM
Status update03/14/2023 3:43:07 PM
Last indexation02/12/2024 6:28:15 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack