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Simultaneous detection of parainfluenza viruses 1 and 3 by real-time reverse transcription-polymerase chain reaction

Published in Journal of virological methods. 2009, vol. 156, no. 1-2, p. 166-168
Abstract Human parainfluenza virus (HPIV) types 1 and 3 are major viral pathogens responsible for upper and lower respiratory tract infections. The diagnosis of these two species is achieved generally by specific reverse transcription-polymerase chain (RT-PCR) reaction methods. In this study, a real-time RT-PCR was developed using a common pair of primers-probe (HPIV-1+3) for the simultaneous detection of both HPIV-1 and HPIV-3 genomes. Results obtained in a 10-fold dilution series assay demonstrate a high sensitivity of the assay with a lowest detection limit of approximately one plasmid copy for both HPIV-1 and HPIV-3. A comparison of HPIV-1 and HPIV-3 clinical sample detection between specific HPIV-1/HPIV-3 pairs of primers-probes and the HPIV-1+3 combination clearly shows that the latter is significantly more sensitive (gain of about five threshold cycles) than the former for HPIV-3 detection, while equivalent values are observed for HPIV-1. The HPIV-1+3 combination constitutes a more rapid, more sensitive, and less expensive alternative than classical or multiplex real-time RT-PCR assays usually used in clinical laboratories.
Keywords DNA PrimersHumansParainfluenza Virus 1, Human/genetics/ isolation & purificationParainfluenza Virus 3, Human/genetics/ isolation & purificationRNA, Viral/analysisRespirovirus Infections/ diagnosisReverse Transcriptase Polymerase Chain Reaction/ methodsSensitivity and Specificity
PMID: 19063922
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Research group Groupe Laurent Kaiser (virologie clinique) (668)
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CORDEY, Samuel et al. Simultaneous detection of parainfluenza viruses 1 and 3 by real-time reverse transcription-polymerase chain reaction. In: Journal of virological methods, 2009, vol. 156, n° 1-2, p. 166-168. doi: 10.1016/j.jviromet.2008.11.006 https://archive-ouverte.unige.ch/unige:7153

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Deposited on : 2010-06-21

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