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Scientific article
English

Recent developments in liquid chromatography--impact on qualitative and quantitative performance

Contributors

Guillarme, Davy; Nguyen, Dao T-T.; Rudaz, Serge; Veuthey, Jean-Luc

Published in

Journal of chromatography, vol. 1149, no. 1, p. 20-29

Publication date

2007

Abstract

In order to reduce the analysis time and maintain good efficiency in liquid chromatography (LC), several solutions are currently being investigated. The focus of this study was to compare, both qualitatively and quantitatively, the chromatographic performance of a conventional LC with selected approaches, namely monolithic supports, high temperature LC (up to 90 degrees C), and sub-2 microm particles combined with high pressure (up to 1000 bar). This comparison was achieved from a qualitative point of view with a special attention paid to the analysis of time reduction, efficiency improvement, and pressure constraint. For this purpose, the different approaches were discussed using Knox curves and other kinetic plots. It appeared that columns packed with sub-2 microm particles under high-pressure conditions (UPLC) were well adapted and this option represents an attractive alternative to conventional LC; however, the other alternative approaches should not be neglected. The quantitative evaluation of these techniques was performed on the basis of the validation of results of a pharmaceutical formulation (Rapidocaïne), following SFSTP 2003 guidelines. Fast-LC approaches demonstrated equivalent performance to conventional LC in terms of trueness, precision, and accuracy profile, with a significant time reduction (up to 8x) according to the selected methodology.

Keywords

  • Chromatography, Liquid/methods
  • Kinetics
  • Particle Size
  • Reproducibility of Results
  • Temperature

UNIGE affiliation entities

Citation (ISO format)

GUILLARME, Davy et al. Recent developments in liquid chromatography--impact on qualitative and quantitative performance. In: Journal of chromatography, 2007, vol. 1149, n° 1, p. 20–29. doi: 10.1016/j.chroma.2006.11.014

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0021-9673
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Technical informations

Creation

15/06/2010 13:26:00

First validation

15/06/2010 13:26:00

Update

14/03/2023 15:30:03

Status update

14/03/2023 15:30:03

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