UNIGE document Scientific Article
previous document  unige:6871  next document
add to browser collection

Recent developments in liquid chromatography--impact on qualitative and quantitative performance

Nguyen, Dao T-T.
Published in Journal of chromatography. 2007, vol. 1149, no. 1, p. 20-9
Abstract In order to reduce the analysis time and maintain good efficiency in liquid chromatography (LC), several solutions are currently being investigated. The focus of this study was to compare, both qualitatively and quantitatively, the chromatographic performance of a conventional LC with selected approaches, namely monolithic supports, high temperature LC (up to 90 degrees C), and sub-2 microm particles combined with high pressure (up to 1000 bar). This comparison was achieved from a qualitative point of view with a special attention paid to the analysis of time reduction, efficiency improvement, and pressure constraint. For this purpose, the different approaches were discussed using Knox curves and other kinetic plots. It appeared that columns packed with sub-2 microm particles under high-pressure conditions (UPLC) were well adapted and this option represents an attractive alternative to conventional LC; however, the other alternative approaches should not be neglected. The quantitative evaluation of these techniques was performed on the basis of the validation of results of a pharmaceutical formulation (Rapidocaïne), following SFSTP 2003 guidelines. Fast-LC approaches demonstrated equivalent performance to conventional LC in terms of trueness, precision, and accuracy profile, with a significant time reduction (up to 8x) according to the selected methodology.
Keywords Chromatography, Liquid/methodsKineticsParticle SizeReproducibility of ResultsTemperature
PMID: 17129584
Full text
Article (Published version) (627 Kb) - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
GUILLARME, Davy et al. Recent developments in liquid chromatography--impact on qualitative and quantitative performance. In: Journal of chromatography, 2007, vol. 1149, n° 1, p. 20-9. doi: 10.1016/j.chroma.2006.11.014 https://archive-ouverte.unige.ch/unige:6871

483 hits

1 download


Deposited on : 2010-06-15

Export document
Format :
Citation style :