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Scientific article
English

Central composite design in the chiral analysis of amphetamines by capillary electrophoresis

Contributors

Varesio, Emmanuel; Gauvrit, Jean-Yves; Longeray, Rémi; Lantéri, Pierre; Veuthey, Jean-Luc

Published in

Electrophoresis, vol. 18, no. 6, p. 931-937

Publication date

1997

Abstract

We have studied a central composite design for the chiral separation of amphetamines using capillary electrophoresis. Five variables, i.e., buffer concentration, pH, chiral selector concentration, temperature, and applied voltage, were investigated. Enantiomeric resolutions as well as analysis time and generated power were established as responses for each experiment. A model of each response was obtained by multiple regression of a quadratic-degree mathematical expression. From the models, we determined the most favorable conditions for the chiral separation by optimizing the resolution and setting the other responses at threshold values. Results were compared with a previous study in which a systematic investigation of the operating parameters was carried out. In order to visualize the robustness of the method, response surfaces were drawn for the significant variables. We have concluded that experimental designs offer a rapid means of optimizing several variables and provide an efficient test for the robustness of the analytical method.

Keywords

  • Amphetamine/analysis
  • Electrophoresis, Capillary/methods
  • Molecular Conformation
  • Regression Analysis

UNIGE affiliation entities

Citation (ISO format)

VARESIO, Emmanuel et al. Central composite design in the chiral analysis of amphetamines by capillary electrophoresis. In: Electrophoresis, 1997, vol. 18, n° 6, p. 931–937. doi: 10.1002/elps.1150180613

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0173-0835
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Technical informations

Creation

15/06/2010 14:45:00

First validation

15/06/2010 14:45:00

Update

14/03/2023 15:30:03

Status update

14/03/2023 15:30:03

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