Scientific article

Determination of dexamethasone in tears by capillary electrophoresis

Publication date1997

A selective capillary zone electrophoresis (CZE) microassay was developed for the simultaneous determination of dexamethasone phosphate and its major metabolite, dexamethasone, in tears. The calibration was carried out in the biological matrix with indoprofen as an internal standard which allowed the separation of dexamethasone phosphate and dexamethasone from the tear constituents. The limits of detection and quantification of the assay were 0.5 and 2.0 microg ml(-1), respectively. This quantification method is essential for the in vivo determination of dexamethasone concentration-time profiles in tears after application of the antiinflammatory drug.

  • Administration, Topical
  • Anti-Inflammatory Agents/analysis
  • Dexamethasone/analysis
  • Electrophoresis, Capillary
  • Glucocorticoids/analysis
  • Sensitivity and Specificity
  • Tears/chemistry
Citation (ISO format)
BAEYENS, V. et al. Determination of dexamethasone in tears by capillary electrophoresis. In: Journal of chromatography. B, Biomedical sciences and applications, 1997, vol. 692, n° 1, p. 222–226.
ISSN of the journal1387-2273

Technical informations

Creation06/15/2010 2:48:00 PM
First validation06/15/2010 2:48:00 PM
Update time03/14/2023 3:30:02 PM
Status update03/14/2023 3:30:02 PM
Last indexation08/28/2023 6:06:37 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack