High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature

Nguyen, Dao T-T.; Guillarme, Davy; Heinisch, Sabine; Barrioulet, Marie-Pierre; Rocca, Jean-Louis; Rudaz, Serge; Veuthey, Jean-Luc

doi:10.1016/j.chroma.2007.08.032

Scientific article

English

High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature

Contributors

Nguyen, Dao T-T.; Guillarme, Davy; Heinisch, Sabine; Barrioulet, Marie-Pierre; Rocca, Jean-Louis; Rudaz, Serge

; Veuthey, Jean-Luc

Published in

Journal of chromatography, vol. 1167, no. 1, p. 76-84

Publication date

2007

Abstract

In this study, ultra performance liquid chromatography (UPLC) using pressures up to 1,000 bar and columns packed with sub-2 microm particles has been combined with high temperature mobile phase conditions (up to 90 degrees C). By using high temperature ultra performance liquid chromatography (HT-UPLC), it is possible to drastically decrease the analysis time without loss in efficiency. The stability and chromatographic behavior of sub-2 microm particles were evaluated at high temperature and high pressure. The chromatographic support remained stable after 500 injections (equivalent to 7,500 column volumes) and plate height curves demonstrated the capability of HT-UPLC to obtain fast separations. For example, a separation of nine doping agents was performed in less than 1 min with sub-2 microm particles at 90 degrees C. Furthermore, a shorter column (30 mm length) was used and allowed a separation of eight pharmaceutical compounds in only 40s.

Keywords

Chromatography, High Pressure Liquid/instrumentation/methods
Hot Temperature
Kinetics
Models, Chemical
Particle Size
Pharmaceutical Preparations/chemistry/isolation & purification
Porosity
Pressure
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet/methods
Temperature
Time Factors

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

NGUYEN, Dao T-T. et al. High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature. In: Journal of chromatography, 2007, vol. 1167, n° 1, p. 76–84. doi: 10.1016/j.chroma.2007.08.032

Article (Published version)

Identifiers

PID : unige:6864
DOI : 10.1016/j.chroma.2007.08.032
PMID : 17765255

Journal ISSN

0021-9673

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15/06/2010 13:37:00

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High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation