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Title

High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature
Authors
Nguyen, Dao T-T.
Guillarme, Davy
Heinisch, Sabine
Barrioulet, Marie-Pierre
Rocca, Jean-Louis
Rudaz, Serge
Veuthey, Jean-Luc
Published in Journal of Chromatography. A. 2007, vol. 1167, no. 1, p. 76-84
Abstract In this study, ultra performance liquid chromatography (UPLC) using pressures up to 1,000 bar and columns packed with sub-2 microm particles has been combined with high temperature mobile phase conditions (up to 90 degrees C). By using high temperature ultra performance liquid chromatography (HT-UPLC), it is possible to drastically decrease the analysis time without loss in efficiency. The stability and chromatographic behavior of sub-2 microm particles were evaluated at high temperature and high pressure. The chromatographic support remained stable after 500 injections (equivalent to 7,500 column volumes) and plate height curves demonstrated the capability of HT-UPLC to obtain fast separations. For example, a separation of nine doping agents was performed in less than 1 min with sub-2 microm particles at 90 degrees C. Furthermore, a shorter column (30 mm length) was used and allowed a separation of eight pharmaceutical compounds in only 40s.
Keywords Chromatography, High Pressure Liquid/instrumentation/methods — Hot Temperature — Kinetics — Models, Chemical — Particle Size — Pharmaceutical Preparations/chemistry/isolation & purification — Porosity — Pressure — Reproducibility of Results — Sensitivity and Specificity — Spectrophotometry, Ultraviolet/methods — Temperature — Time Factors
Identifiers
PMID: 17765255
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Article (Published version) (663 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Faculté des sciences / Section des sciences pharmaceutiques
Citation
(ISO format) 		NGUYEN, Dao T-T. et al. High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature. In: Journal of Chromatography. A, 2007, vol. 1167, n° 1, p. 76-84. https://archive-ouverte.unige.ch/unige:6864

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Deposited on : 2010-06-15

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