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High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature

Nguyen, Dao T-T.
Heinisch, Sabine
Barrioulet, Marie-Pierre
Rocca, Jean-Louis
Published in Journal of chromatography. 2007, vol. 1167, no. 1, p. 76-84
Abstract In this study, ultra performance liquid chromatography (UPLC) using pressures up to 1,000 bar and columns packed with sub-2 microm particles has been combined with high temperature mobile phase conditions (up to 90 degrees C). By using high temperature ultra performance liquid chromatography (HT-UPLC), it is possible to drastically decrease the analysis time without loss in efficiency. The stability and chromatographic behavior of sub-2 microm particles were evaluated at high temperature and high pressure. The chromatographic support remained stable after 500 injections (equivalent to 7,500 column volumes) and plate height curves demonstrated the capability of HT-UPLC to obtain fast separations. For example, a separation of nine doping agents was performed in less than 1 min with sub-2 microm particles at 90 degrees C. Furthermore, a shorter column (30 mm length) was used and allowed a separation of eight pharmaceutical compounds in only 40s.
Keywords Chromatography, High Pressure Liquid/instrumentation/methodsHot TemperatureKineticsModels, ChemicalParticle SizePharmaceutical Preparations/chemistry/isolation & purificationPorosityPressureReproducibility of ResultsSensitivity and SpecificitySpectrophotometry, Ultraviolet/methodsTemperatureTime Factors
PMID: 17765255
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NGUYEN, Dao T-T. et al. High throughput liquid chromatography with sub-2 microm particles at high pressure and high temperature. In: Journal of chromatography, 2007, vol. 1167, n° 1, p. 76-84. doi: 10.1016/j.chroma.2007.08.032 https://archive-ouverte.unige.ch/unige:6864

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Deposited on : 2010-06-15

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