UNIGE document Scientific Article
previous document  unige:6857  next document
add to browser collection
Title

New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches

Authors
Published in Analytical and Bioanalytical Chemistry. 2010, vol. 397, no. 3, p. 1069-82
Collection Open Access - Licence nationale Springer
Abstract Recent developments in chromatographic supports and instrumentation for liquid chromatography (LC) are enabling rapid and highly efficient separations. Various analytical strategies have been proposed, for example the use of silica-based monolithic supports, elevated mobile phase temperatures, and columns packed with sub-3 microm superficially porous particles (fused core) or with sub-2 microm porous particles for use in ultra-high-pressure LC (UHPLC). The purpose of this review is to describe and compare these approaches in terms of throughput and resolving power, using kinetic data gathered for compounds with molecular weights ranging between 200 and 1300 g mol(-1) in isocratic and gradient modes. This study demonstrates that the best analytical strategy should be selected on the basis of the analytical problem (e.g., isocratic vs. gradient, throughput vs. efficiency) and the properties of the analyte. UHPLC and fused-core technologies are quite promising for small-molecular-weight compounds, but increasing the mobile phase temperature is useful for larger molecules, for example peptides.
Keywords UHPLCUPLCHTLCMonolithFused-coreKinetic plots
Identifiers
PMID: 19998028
Full text
Structures
Citation
(ISO format)
GUILLARME, Davy et al. New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches. In: Analytical and Bioanalytical Chemistry, 2010, vol. 397, n° 3, p. 1069-82. https://archive-ouverte.unige.ch/unige:6857

241 hits

7 downloads

Update

Deposited on : 2010-06-14

Export document
Format :
Citation style :