New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches

Guillarme, Davy; Ruta, Josephine; Rudaz, Serge; Veuthey, Jean-Luc

doi:10.1007/s00216-009-3305-8

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Title		New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches
Authors		Guillarme, Davy Ruta, Josephine Rudaz, Serge Veuthey, Jean-Luc
Published in		Analytical and Bioanalytical Chemistry. 2010, vol. 397, no. 3, p. 1069-82
Collection		Open Access - Licence nationale Springer
Abstract		Recent developments in chromatographic supports and instrumentation for liquid chromatography (LC) are enabling rapid and highly efficient separations. Various analytical strategies have been proposed, for example the use of silica-based monolithic supports, elevated mobile phase temperatures, and columns packed with sub-3 microm superficially porous particles (fused core) or with sub-2 microm porous particles for use in ultra-high-pressure LC (UHPLC). The purpose of this review is to describe and compare these approaches in terms of throughput and resolving power, using kinetic data gathered for compounds with molecular weights ranging between 200 and 1300 g mol(-1) in isocratic and gradient modes. This study demonstrates that the best analytical strategy should be selected on the basis of the analytical problem (e.g., isocratic vs. gradient, throughput vs. efficiency) and the properties of the analyte. UHPLC and fused-core technologies are quite promising for small-molecular-weight compounds, but increasing the mobile phase temperature is useful for larger molecules, for example peptides.
Keywords		UHPLC — UPLC — HTLC — Monolith — Fused-core — Kinetic plots
Identifiers		DOI: 10.1007/s00216-009-3305-8 PMID: 19998028
Full text		Article (Published version) (2.3 MB) - Free access Other version: http://www.springerlink.com/content/r190813v56ww08w3/fulltext.pdf
Structures		Faculté des sciences / Section des sciences pharmaceutiques
Citation (ISO format)		GUILLARME, Davy et al. New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches. In: Analytical and Bioanalytical Chemistry, 2010, vol. 397, n° 3, p. 1069-82. doi: 10.1007/s00216-009-3305-8 https://archive-ouverte.unige.ch/unige:6857