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Scientific article
English

Use of monolithic supports for fast analysis of drugs and metabolites in plasma by direct injection

Published inChromatographia, vol. 57, no. 9-10, p. 569-575
Publication date2003
Abstract

Commercial monolithic columns were evcluated as extraction supports for the analysis of various drugs and their metabolites from plasma. For this purpose, a precolumn packed with a monolithic phase was used as extraction and enrichment support and integrated in a column-switching system for the direct and simultaneous analysis of fluoxetine and norfluoxetine, methadone and EDDP, flunitrazepam and norflunitrazepom in humon plasma. In this configuration, plasma samples were directly injected onto a Chromolith Flash precolumn (25×4.6 mm I.D.) coupled to a microbore analytical column packed with a conventional reversed-phase malerial. Detection was by an electrospray-ionization mass spectrometer (ESl-MS). Total analysis time, including extraction and separation of the six analytes, was <10 min. For all the investigated compounds, the limits of quantitation (LOQ) were estimated in the ng.mL1 range for an injection volume of 50 μL. The method was also applied to a real clinical case to demonstrate its applicability.

Keywords
  • Column liquid chromatography
  • Monolithic columns
  • Drugs and metabolites
  • Fast analysis
Citation (ISO format)
SOUVERAIN, Sandrine, RUDAZ, Serge, VEUTHEY, Jean-Luc. Use of monolithic supports for fast analysis of drugs and metabolites in plasma by direct injection. In: Chromatographia, 2003, vol. 57, n° 9-10, p. 569–575. doi: 10.1007/BF02491731
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ISSN of the journal1612-1112
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