Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part I

Stella, Cinzia; Rudaz, Serge; Veuthey, Jean-Luc; Tchapla, A.

doi:10.1007/BF02490318

Scientific article

English

Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part I

Contributors

Stella, Cinzia; Rudaz, Serge

; Veuthey, Jean-Luc; Tchapla, A.

Published in

Chromatographia, vol. 53, no. S1, p. S113-S131

Publication date

2001

Abstract

Reversed-phase liquid chromatography (RP-HPLC) has become a powerful and widely employed technique in the separation and analysis of a great variety of compounds with different functionalities. The most common type of stationary phase for RP-HPLC consists of nonpolar, hydrophobic organic species (e.g., octyl, octadecyl) attached by siloxane bonds to the surface of a silica support. In the first part of this article, a description of the many beneficial properties that make porous silica the most employed support in RP-HPLC will be presented, starting from the synthesis of silica. It is noteworthy that the chromatographic properties of the final column are strictly correlated to the preparation type. A silica surface possesses a number of attractive properties, but also some drawbacks. Unreacted or residual silanols interact with basic compounds and can induced peak tailing, which means a loss in chromatographic performance. This problem has lead many manufactures to produce stationary phases with reduced silanol activity which improve dramatically the peak shape of basic compounds. In the second part of this review, different approaches are proposed to obtain less reactive stationary phases.

Keywords

Column liquid chromatography
Stationary phases
Silica
Review

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

STELLA, Cinzia et al. Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part I. In: Chromatographia, 2001, vol. 53, n° S1, p. S113–S131. doi: 10.1007/BF02490318

Article (Published version)

Identifiers

PID : unige:6739
DOI : 10.1007/BF02490318

Additional URL for this publication

http://www.springerlink.com/index/10.1007/BF02490318

Journal ISSN

0009-5893

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31/05/2010 15:54:00

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26/03/2026 10:49:34

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Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part I

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation