Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part II

Stella, Cinzia; Rudaz, Serge; Veuthey, Jean-Luc; Tchapla, A.

doi:10.1007/BF02490319

Scientific article

English

Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part II

Contributors

Stella, Cinzia; Rudaz, Serge

; Veuthey, Jean-Luc; Tchapla, A.

Published in

Chromatographia, vol. 53, no. S1, p. S132-S140

Publication date

2001

Abstract

Reversed-phase chromatography is the method of choice for the analysis of basic compounds. However, because of the problems encountered with these compounds, “base deactivated” stationary phases have been developed. This review focuses on a major problem encountered by the chromatographer which is to effectively choose, for a particular separation, the appropriate reversed-phase packing. Often, columns of the same type but provided by various manufacturers vary in their chromatographic behaviour. It is known that differences in the purity of column packings, in the properties of silica gel supports and the density of silanols on the surface can be responsible for chromatographic differences. Unfortunately, in the case of “base deactivated” columns there is no universal test and chromatographic effects are compound dependent. The aim of this review is to describe the various chromatographic tests proposed in the literature for the differentiation of the many stationary phases on the market.

Keywords

Column liquid chromatography
Chromatographic tests
Basic compounds
Review

UNIGE affiliation entities

Faculté des sciences / Section des sciences pharmaceutiques

Citation (ISO format)

STELLA, Cinzia et al. Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part II. In: Chromatographia, 2001, vol. 53, n° S1, p. S132–S140. doi: 10.1007/BF02490319

Article (Published version)

Identifiers

PID : unige:6738
DOI : 10.1007/BF02490319

Additional URL for this publication

http://www.springerlink.com/index/10.1007/BF02490319

Journal ISSN

0009-5893

610views

1downloads

Creation

31/05/2010 16:44:00

First validation

31/05/2010 16:44:00

Update

26/03/2026 10:49:26

Status update

26/03/2026 10:49:26

Last indexation

26/03/2026 10:56:02

Silica and other materials as supports in liquid chromatography. Chromatographic tests and their importance for evaluating these supports. Part II

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation