UNIGE document Scientific Article
previous document  unige:6710  next document
add to browser collection
Title

Rapid stereoselective separations of amphetamine derivatives with highly sulfated gamma-cyclodextrin

Authors
Souverain, Sandrine
Published in Electrophoresis. 2005, vol. 26, no. 20, p. 3910-20
Abstract The highly sulfated gamma-CD (HS-gamma-CD) is a chiral selector widely used in CE for the enantioseparation of pharmaceutical compounds. This paper investigated different approaches to reduce the stereoselective analysis time of amphetamine (AT) derivatives according to the chiral selector concentration in the BGE. With high HS-gamma-CD concentration, tested analytes were separated in 3.5 min as anionic complexes with short-end injection technique in reversed polarity mode. However, this procedure presented some limitations in terms of efficiency and resolution, excessive Joule heating and poor compatibility with MS detection. With low HS-gamma-CD concentration, compounds were separated as cations. Conventional approaches to reduce CE analysis time demonstrated critical resolution between some analytes. Therefore, the use of the partial-filling technique compatible with MS detection was carried out. Under optimized conditions, the analysis time for the chiral separation of seven AT like compounds was reduced to 6 min. Moreover, sensitivity of CE-MS was sufficient for the determination of ATs in plasma following a simple liquid-liquid extraction.
Keywords Amphetamines/blood/isolation & purificationCationsElectrophoresis, Capillary/methodsHumansStereoisomerismSulfatesTime FactorsGamma-Cyclodextrins
Identifiers
PMID: 16167308
Full text
Article (Published version) (241 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
RUDAZ, Serge et al. Rapid stereoselective separations of amphetamine derivatives with highly sulfated gamma-cyclodextrin. In: Electrophoresis, 2005, vol. 26, n° 20, p. 3910-20. https://archive-ouverte.unige.ch/unige:6710

221 hits

3 downloads

Update

Deposited on : 2010-05-28

Export document
Format :
Citation style :