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Determination of electroosmotic flow in nonaqueous capillary electrophoresis

Mirgaldi, Myriam
Published in Journal of chromatography. 2005, vol. 1068, no. 1, p. 75-81
Abstract Mobility of the electroosmotic flow (mu(EOF)) in fused-silica capillaries strongly depends on the nature of the background electrolyte. In this study, 27 solvent systems were investigated, namely water, methanol, ethanol, 2-propanol, 1-butanol, acetonitrile (MeCN), formamide, N-methylformamide (NMF), N,N-dimethylformamide and dimethyl sulfoxyde, as well as 8 hydroorganic and 9 organic mixtures. For each system, six mu(EOF) were determined at a different ionic strength in basic conditions, and an absolute electroosmotic flow mobility (mu(EOF,0)) was extrapolated according to the Debye-Huckel Onsager model. The obtained mu(EOF,0) values were correlated with the solvent's relative permittivity (epsilon) and viscosity (eta). A good correlation (r2=0.867) between mu(EOF,0) and the solvent's epsilon/eta ratio was demonstrated, except for two solvents (MeCN and NMF). Furthermore, the donor number (DN) of a solvent took into account the possible zeta potential modification in the electric double layer near the capillary wall. Consequently, the relationship between mu(EOF,0) and epsilon/(eta x DN) was superior, with a r2 of 0.943 for 10 pure solvents.
Keywords ElectrophoresisCapillary/methodsOsmosisSolvents/chemistry
PMID: 15844544
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GEISER, Laurent, MIRGALDI, Myriam, VEUTHEY, Jean-Luc. Determination of electroosmotic flow in nonaqueous capillary electrophoresis. In: Journal of chromatography, 2005, vol. 1068, n° 1, p. 75-81. doi: 10.1016/j.chroma.2005.02.001 https://archive-ouverte.unige.ch/unige:6533

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Deposited on : 2010-05-07

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