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Nanoscale liquid chromatography and capillary electrophoresis coupled to electrospray mass spectrometry for the detection of amyloid-β peptide related to alzheimer's disease

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Published in Journal of Chromatography. A. 2002, vol. 974, p. 135-142
Abstract Alzeihmer's disease (AD) is a neurodegenerative disorder which is pathologically characterized by the progressive deposit in the brain of a specific form of amyloid, amyloid-β peptides (Aβ). As the latter circulate in the blood, their quantitation in plasma could allow a simple diagnosis of AD. Aβ are present in different variants, one of which contains 40 amino acid residues (Aβ1-40). In this work, nanoscale liquid chromatography (nano-LC) and capillary electrophoresis (CE) coupled to mass spectrometry (MS) were compared to determine the most appropriate technique for reaching the usual Aβ1-40 concentration in plasma or serum. Both a 50 μm I.D. CE capillary and a 75 μm I.D. nano-LC column were coupled to a single quadrupole mass spectrometer with a sheath-liquid electrospray (ESI) interface or a homemade nanospray interface, respectively. Capillary zone electrophoresis is a powerful separation technique, but its low sensitivity limits its use in the analysis of biological matrices. However, a column-switching set-up with a precolumn of 1 mm×300 μm I.D. packed with a C18 PepMap (3 μm) stationary phase and a nanocolumn of 15 cm×75 μm I.D. packed with the same stationary phase was found to be a successful technique which allowed detection of Aβ1-40 at the ng ml-1 level (a few hundred femtomoles injected) because of its higher sample loading capability.
Keywords Degenerative diseaseCerebral disorderCentral nervous system diseaseNervous system diseasesAlzheimer diseaseElectrosprayMass spectrometryCoupled methodCapillary electrophoresisHPLC chromatographyNanometer scaleMicroanalysisMicroassayBloodBlood plasmaBlood serumBiological fluidPatientDiagnosisβ Amyloid proteinPeptidesPeptide fragmentQualitative analysisChemical analysisAnalysis method
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VARESIO, Emmanuel et al. Nanoscale liquid chromatography and capillary electrophoresis coupled to electrospray mass spectrometry for the detection of amyloid-β peptide related to alzheimer's disease. In: Journal of Chromatography. A, 2002, vol. 974, p. 135-142. https://archive-ouverte.unige.ch/unige:6531

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Deposited on : 2010-05-07

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