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New extraction supports for the fast analysis of pharmaceutical compounds in biological samples by direct injection

Souverain, Sandrine
Published in GIT laboratory journal Europe. 2003, vol. 7, no. 2, p. 80-82
Abstract The number of samples to be analyzed during the drug discovery process is constantly increasing. Therefore, fast, selective and sensitive analytical methods are required. Nowadays, liquid chromatography coupled with mass spectrometry (LC-MS) is considered as the method of choice. With this performant tool, the sample preparation step prior to analysis can be simplified and the analytical process speeded up. Therefore, several sample extraction techniques, coupled on-line with a LC-MS system, have recently been developed. In this work, different extraction supports allowing the direct injection of biological fluids were investigated. In the single column mode or in the column switching configuration, these supports were found suitable for the fast analysis (less than 15 min) of drugs and their metabolites present at ng.ml-1 level in biological matrices.
Keywords Monolithic columnRestricted access medium columnElectrosprayMass spectrometryHPLC chromatographyMetaboliteFlunitrazepamSedativeBenzodiazepine derivativesFluoxetinePsychotropicAntidepressant agentNarcotic analgesicOpiatesMethadoneBloodBlood plasmaBlood serumBiological fluidOn lineColumn switchingCoupled methodAdsorbentSolid phase extractionSample preparationRapid techniqueTrace analysisChemical analysis
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SOUVERAIN, Sandrine, RUDAZ, Serge, VEUTHEY, Jean-Luc. New extraction supports for the fast analysis of pharmaceutical compounds in biological samples by direct injection. In: GIT laboratory journal Europe, 2003, vol. 7, n° 2, p. 80-82. https://archive-ouverte.unige.ch/unige:6445

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Deposited on : 2010-05-03

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