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Title

Characterization and comparison of the chromatographic performance of different types of reversed-phase stationary phases

Authors
Stella, Cinzia
Gauvrit, Jean-Yves
Lantéri, Pierre
Huteau, Alban
Tchapla, Alain
Published in Journal of Pharmaceutical and Biomedical Analysis. 2007, vol. 43, no. 1, p. 89-98
Abstract The chromatographic performance of several base-deactivated stationary phases was evaluated with a specific chromatographic test. Seven basic test compounds, possessing different physico-chemical properties were injected on different supports with two mobile phases: one at pH 7.0 (acetonitrile-phosphate buffer, 40:60, v/v), and the other at pH 3.0 (acetonitrile-phosphate buffer, 15:85, v/v). Chromatographic parameters obtained under these conditions were treated by principal component analysis (PCA) to separate base deactivated supports according to their silanol activity (pH 7.0 mobile phase) and hydrophobic properties (pH 3.0 mobile phase). The information given by the specific test column evaluation was improved with complementary chemometric tools such as hierarchical cluster analysis. The same base deactivated supports were also tested following a general test procedure issued from the literature and obtained fundamental properties (in particular silanol activity and hydrophobicity) were compared with column evaluation obtained with the specific test: results were in good agreement, although the use of the specific test offered a better differentiation between numerous base-deactivated supports.
Keywords Chemistry, PhysicalChromatography/instrumentation/statistics & numerical dataCluster AnalysisHydrogen-Ion ConcentrationIndicators and ReagentsParticle SizePhysicochemical PhenomenaPrincipal Component AnalysisReproducibility of ResultsSilanes/chemistrySoftware
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PMID: 16860511
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STELLA, Cinzia et al. Characterization and comparison of the chromatographic performance of different types of reversed-phase stationary phases. In: Journal of Pharmaceutical and Biomedical Analysis, 2007, vol. 43, n° 1, p. 89-98. doi: 10.1016/j.jpba.2006.06.018 https://archive-ouverte.unige.ch/unige:6444

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Deposited on : 2010-05-03

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