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Identification of CIITA regulated genetic module dedicated for antigen presentation

Sperisen, Peter
Schmid, Christoph
Bucher, Philipp
Published in PLOS genetics. 2008, vol. 4, no. 4, e1000058
Abstract The class II trans-activator CIITA is a transcriptional co-activator required for the expression of Major Histocompatibility Complex (MHC) genes. Although the latter function is well established, the global target-gene specificity of CIITA had not been defined. We therefore generated a comprehensive list of its target genes by performing genome-wide scans employing four different approaches designed to identify promoters that are occupied by CIITA in two key antigen presenting cells, B cells and dendritic cells. Surprisingly, in addition to MHC genes, only nine new targets were identified and validated by extensive functional and expression analysis. Seven of these genes are known or likely to function in processes contributing to MHC-mediated antigen presentation. The remaining two are of unknown function. CIITA is thus uniquely dedicated for genes implicated in antigen presentation. The finding that CIITA regulates such a highly focused gene expression module sets it apart from all other transcription factors, for which large-scale binding-site mapping has indicated that they exert pleiotropic functions and regulate large numbers of genes.
Keywords B-LymphocytesBase SequenceBinding SitesCell LineChromatin ImmunoprecipitationDNADNA-Binding ProteinsDendritic CellsEnhancer Elements (Genetics)GenesMHC Class IIHumansInterferon-gamma RecombinantNuclear ProteinsPromoter Regions (Genetics)Trans-Activation (Genetics)Trans-ActivatorsTranscription Factors
PMID: 18437201
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Article (Accepted version) (742 Kb) - public document Free access
Research group Groupe Reith Walter (pathologie et immunologie) (282)
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KRAWCZYK, Michal et al. Identification of CIITA regulated genetic module dedicated for antigen presentation. In: PLOS genetics, 2008, vol. 4, n° 4, p. e1000058. doi: 10.1371/journal.pgen.1000058 https://archive-ouverte.unige.ch/unige:639

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Deposited on : 2008-12-09

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