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The dual control of TFIIB recruitment by NC2 is gene specific

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Published in Nucleic Acids Research. 2008, vol. 36, no. 2, p. 539-49
Abstract Negative co-factor 2 (NC2) is a conserved eukaryotic complex composed of two subunits, NC2alpha (Drap1) and NC2beta (Dr1) that associate through a histone-fold motif. In this work, we generated mutants of NC2, characterized target genes for these mutants and studied the assembly of NC2 and general transcription factors on target promoters. We determined that the two NC2 subunits mostly function together to be recruited to DNA and regulate gene expression. We found that NC2 strongly controls promoter association of TFIIB, both negatively and positively. We could attribute the gene-specific repressor effect of NC2 on TFIIB to the C-terminal domain of NC2beta, and define that it requires ORF sequences of the target gene. In contrast, the positive function of NC2 on TFIIB targets is more general and requires adequate levels of the NC2 histone-fold heterodimer on promoters. Finally, we determined that NC2 becomes limiting for TATA-binding protein (TBP) association with a heat inducible promoter under heat stress. This study demonstrates an important positive role of NC2 for formation of the pre-initiation complex on promoters, under normal conditions through control of TFIIB, or upon activation by stress via control of TBP.
Keywords Gene Expression RegulationHeat-Shock ProteinsHeat-Shock ResponseMutationOligonucleotide Array Sequence AnalysisPhosphoproteinsPromoter Regions (Genetics)Protein StructureTertiaryProtein SubunitsRNAMessengerSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription Factor TFIIBTranscription FactorsTranscriptionGenetic
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PMID: 18048413
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Article (Published version) (3.7 MB) - public document Free access
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Research group Groupe Collart Martine (microbiologie et médecine moléculaire) (81)
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MASSON, Patrick Marc David et al. The dual control of TFIIB recruitment by NC2 is gene specific. In: Nucleic Acids Research, 2008, vol. 36, n° 2, p. 539-49. https://archive-ouverte.unige.ch/unige:605

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Deposited on : 2008-11-26

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