The dual control of TFIIB recruitment by NC2 is gene specific

Article Article scientifique Article The dual control of TFIIB recruitment by NC2 is gene specific eng 2008 author Masson Patrick Marc David 601638 author Leimgruber Elisa 510528 author Creton Sandrine Yolande Germaine 522701 author Collart Martine 0000-0001-6955-1773 27009 539-549 10.1093/nar/gkm1078 18048413 0305-1048 Negative co-factor 2 (NC2) is a conserved eukaryotic complex composed of two subunits, NC2alpha (Drap1) and NC2beta (Dr1) that associate through a histone-fold motif. In this work, we generated mutants of NC2, characterized target genes for these mutants and studied the assembly of NC2 and general transcription factors on target promoters. We determined that the two NC2 subunits mostly function together to be recruited to DNA and regulate gene expression. We found that NC2 strongly controls promoter association of TFIIB, both negatively and positively. We could attribute the gene-specific repressor effect of NC2 on TFIIB to the C-terminal domain of NC2beta, and define that it requires ORF sequences of the target gene. In contrast, the positive function of NC2 on TFIIB targets is more general and requires adequate levels of the NC2 histone-fold heterodimer on promoters. Finally, we determined that NC2 becomes limiting for TATA-binding protein (TBP) association with a heat inducible promoter under heat stress. This study demonstrates an important positive role of NC2 for formation of the pre-initiation complex on promoters, under normal conditions through control of TFIIB, or upon activation by stress via control of TBP. Gene Expression Regulation Heat-Shock Proteins Heat-Shock Response Mutation Oligonucleotide Array Sequence Analysis Phosphoproteins Promoter Regions (Genetics) Protein Structure Tertiary Protein Subunits RNA Messenger Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Transcription Factor TFIIB Transcription Factors Transcription Genetic Dewey 616 Dewey 616.07 54 Département de microbiologie et médecine moléculaire 21B 137 Département de pathologie et immunologie 21F false 58 Caractérisation génétique et biochimique du complexe Ccr4-Not conservé 81 Nucleic acids research 36 2 8d465393-c520-43d1-bd81-25c52a28b4ba Article (Published version) Public 18048413.pdf 3910476 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de microbiologie et médecine moléculaire Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie

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The dual control of TFIIB recruitment by NC2 is gene specific	Nucleic acids research			2008	690	464

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Creton, Sandrine Yolande Germaine