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Cytosolic free Ca2+ in insulin secreting cells and its regulation by isolated organelles

Prentki, M
Published in Experientia. 1984, vol. 40, no. 10, p. 1052-60
Abstract The role of Ca2+ in secretagogue-induced insulin release is documented not only by the measurements of 45Ca fluxes in pancreatic islets, but also, by direct monitoring of cytosolic free Ca2+, [Ca2+]i. As demonstrated, using the fluorescent indicator quin 2, glyceraldehyde, carbamylcholine and alanine raise [Ca2+]i in the insulin secreting cell line RINm5F, whereas glucose has a similar effect in pancreatic islet cells. The regulation of cellular Ca2+ homeostasis by organelles from a rat insulinoma, was investigated with a Ca2+ selective electrode. The results suggest that both the endoplasmic reticulum and the mitochondria participate in this regulation, albeit at different Ca2+ concentrations. By contrast, the secretory granules do not appear to be involved in the short-term regulation of [Ca2+]i. Evidence is presented that inositol 1,4,5-trisphosphate, which is shown to mobilize Ca2+ from the endoplasmic reticulum, is acting as an intracellular mediator in the stimulation of insulin release.
Keywords Adenine Nucleotides/metabolismAlanine/pharmacologyAnimalsBiological Transport, ActiveCalcimycin/pharmacologyCalcium/physiologyCytosol/metabolismEthers/pharmacologyGlucose/pharmacologyGlyceraldehyde/pharmacologyHumansInositol 1,4,5-TrisphosphateInositol Phosphates/physiologyInsulin/secretionIonomycinIslets of Langerhans/cytologyMembrane Potentials/drug effectsModels, BiologicalOrganoids/physiologyVerapamil/pharmacology
PMID: 6436050
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PRENTKI, M, WOLLHEIM, Claes. Cytosolic free Ca2+ in insulin secreting cells and its regulation by isolated organelles. In: Experientia, 1984, vol. 40, n° 10, p. 1052-60. doi: 10.1007/bf01971451 https://archive-ouverte.unige.ch/unige:55476

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Deposited on : 2015-04-13

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