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Scientific article
Open access
English

Junctional adhesion molecule-C mediates leukocyte infiltration in response to ischemia reperfusion injury

Published inArteriosclerosis, thrombosis, and vascular biology, vol. 29, no. 10, p. 1509-1515
Publication date2009
Abstract

OBJECTIVE: Junctional adhesion molecule-C (JAM-C) is an adhesion molecule that has multiple roles in inflammation and vascular biology, but many aspects of its functions under pathological conditions are unknown. Here we investigated the role of JAM-C in leukocyte migration in response to ischemia reperfusion (I/R) injury. METHODS AND RESULTS: Pretreatment of mice with soluble JAM-C (sJAM-C), used as a pharmacological blocker of JAM-C-mediated reactions, significantly suppressed leukocyte migration in models of kidney and cremaster muscle I/R injury (39 and 51% inhibition, respectively). Furthermore, in the cremaster muscle model (studied by intravital microscopy), both leukocyte adhesion and transmigration were suppressed in JAM-C-deficient mice (JAM-C(-/-)) and enhanced in mice overexpressing JAM-C in their endothelial cells (ECs). Analysis of JAM-C subcellular expression by immunoelectron microscopy indicated that in I/R-injured tissues, EC JAM-C was redistributed from cytoplasmic vesicles and EC junctional sites to nonjunctional plasma membranes, a response that may account for the role of JAM-C in both leukocyte adhesion and transmigration under conditions of I/R injury. CONCLUSIONS: The findings demonstrate a role for EC JAM-C in mediating leukocyte adhesion and transmigration in response to I/R injury and indicate the existence of a novel regulatory mechanism for redistribution and hence function of EC JAM-C in vivo.

Keywords
  • Animals
  • Cell Adhesion
  • Cell Adhesion Molecules/analysis/physiology
  • Cell Movement
  • Endothelial Cells/metabolism
  • Immunoglobulins/analysis/physiology
  • Kidney/blood supply
  • Leukocytes/physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Muscle, Skeletal/blood supply
  • Reperfusion Injury/pathology
Citation (ISO format)
SCHEIERMANN, Christoph et al. Junctional adhesion molecule-C mediates leukocyte infiltration in response to ischemia reperfusion injury. In: Arteriosclerosis, thrombosis, and vascular biology, 2009, vol. 29, n° 10, p. 1509–1515. doi: 10.1161/ATVBAHA.109.187559
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Identifiers
ISSN of the journal1079-5642
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