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Title

Junctional adhesion molecule-C mediates leukocyte infiltration in response to ischemia reperfusion injury

Authors
Colom, Bartomeu
Patel, Nimesh S A.
Voisin, Mathieu-Benoit
Marrelli, Alessandra
Woodfin, Abigail
Pitzalis, Costantino
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Published in Arteriosclerosis, Thrombosis, and Vascular Biology. 2009, vol. 29, no. 10, p. 1509-15
Abstract OBJECTIVE: Junctional adhesion molecule-C (JAM-C) is an adhesion molecule that has multiple roles in inflammation and vascular biology, but many aspects of its functions under pathological conditions are unknown. Here we investigated the role of JAM-C in leukocyte migration in response to ischemia reperfusion (I/R) injury. METHODS AND RESULTS: Pretreatment of mice with soluble JAM-C (sJAM-C), used as a pharmacological blocker of JAM-C-mediated reactions, significantly suppressed leukocyte migration in models of kidney and cremaster muscle I/R injury (39 and 51% inhibition, respectively). Furthermore, in the cremaster muscle model (studied by intravital microscopy), both leukocyte adhesion and transmigration were suppressed in JAM-C-deficient mice (JAM-C(-/-)) and enhanced in mice overexpressing JAM-C in their endothelial cells (ECs). Analysis of JAM-C subcellular expression by immunoelectron microscopy indicated that in I/R-injured tissues, EC JAM-C was redistributed from cytoplasmic vesicles and EC junctional sites to nonjunctional plasma membranes, a response that may account for the role of JAM-C in both leukocyte adhesion and transmigration under conditions of I/R injury. CONCLUSIONS: The findings demonstrate a role for EC JAM-C in mediating leukocyte adhesion and transmigration in response to I/R injury and indicate the existence of a novel regulatory mechanism for redistribution and hence function of EC JAM-C in vivo.
Keywords AnimalsCell AdhesionCell Adhesion Molecules/analysis/physiologyCell MovementEndothelial Cells/metabolismImmunoglobulins/analysis/physiologyKidney/blood supplyLeukocytes/physiologyMiceMice, Inbred C57BLMice, TransgenicMuscle, Skeletal/blood supplyReperfusion Injury/pathology
Identifiers
PMID: 19574560
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Other version: http://atvb.ahajournals.org/cgi/content/full/29/10/1509
Structures
Research groups Couplage cellulaire et connexines (136)
Molécules d'adhésion et processus de migration cellulaire (167)
L'influence du rôle du cycle circadien sur l'immunité (995)
Citation
(ISO format)
SCHEIERMANN, Christoph et al. Junctional adhesion molecule-C mediates leukocyte infiltration in response to ischemia reperfusion injury. In: Arteriosclerosis, Thrombosis, and Vascular Biology, 2009, vol. 29, n° 10, p. 1509-15. doi: 10.1161/ATVBAHA.109.187559 https://archive-ouverte.unige.ch/unige:5404

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Deposited on : 2010-03-12

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