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Title

HPLC-UV and HPLC-positive-ESI-MS analysis of the diterpenoid fraction from caper spurge (Euphorbia lathyris) seed oil
Authors
Bicchi, C.
Appendino, G.
Cordero, C.
Rubiolo, P.
Ortelli, Didier
Veuthey, Jean-Luc
Published in Phytochemical Analysis. 2001, vol. 12, no. 4, p. 255-262
Abstract Caper spurge (Euphorbia lathyris L.) seed oil contains a series of diterpenoids known as Euphorbia factors, or L-factors, L1-L9. They are esters of several polyols (lathyrol, epoxylathyrol, hydroxylathyrol and ingenol) and account for about 3-5% of the oil. The percentage of ingenol-based L-factors is very low, less than 5% of the diterpenoid fraction, but some of them (factors L5 and L6) are responsible for the irritant and co-carcinogenic activities of the oil. This paper reports an HPLC-UV and HPLC-positive-ESI-MS analysis of the diterpenoid fraction of caper spurge seed oil before and after selective hydrolysis of ingenol-based L-factors. Separation of lathyrane polyols and esters, and ingenol and its esters was achieved using a chromatographic system consisting of a C18 stationary phase and acetonitrile: water as mobile phase. A new macrocyclic constituent, the deoxy Euphorbia factor L1, was identified in the oil.
Keywords Chromatography, High Pressure Liquid/methods — Diterpenes/analysis — Euphorbia/chemistry — Plant Oils/chemistry — Sensitivity and Specificity — Spectrometry, Mass, Electrospray Ionization/methods — Spectrophotometry, Ultraviolet/methods
Identifiers
PMID: 11705332
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Structures
Faculté des sciences / Section des sciences pharmaceutiques
Citation
(ISO format) 		BICCHI, C. et al. HPLC-UV and HPLC-positive-ESI-MS analysis of the diterpenoid fraction from caper spurge (Euphorbia lathyris) seed oil. In: Phytochemical Analysis, 2001, vol. 12, n° 4, p. 255-262. doi: 10.1002/pca.592 https://archive-ouverte.unige.ch/unige:5138

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Deposited on : 2010-02-09

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