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Scientific article
English

HPLC-UV and HPLC-positive-ESI-MS analysis of the diterpenoid fraction from caper spurge (Euphorbia lathyris) seed oil

Contributors

Bicchi, C.; Appendino, G.; Cordero, C.; Rubiolo, P.; Ortelli, Didier; Veuthey, Jean-Luc

Published in

Phytochemical analysis, vol. 12, no. 4, p. 255-262

Publication date

2001

Abstract

Caper spurge (Euphorbia lathyris L.) seed oil contains a series of diterpenoids known as Euphorbia factors, or L-factors, L1-L9. They are esters of several polyols (lathyrol, epoxylathyrol, hydroxylathyrol and ingenol) and account for about 3-5% of the oil. The percentage of ingenol-based L-factors is very low, less than 5% of the diterpenoid fraction, but some of them (factors L5 and L6) are responsible for the irritant and co-carcinogenic activities of the oil. This paper reports an HPLC-UV and HPLC-positive-ESI-MS analysis of the diterpenoid fraction of caper spurge seed oil before and after selective hydrolysis of ingenol-based L-factors. Separation of lathyrane polyols and esters, and ingenol and its esters was achieved using a chromatographic system consisting of a C18 stationary phase and acetonitrile: water as mobile phase. A new macrocyclic constituent, the deoxy Euphorbia factor L1, was identified in the oil.

Keywords

  • Chromatography, High Pressure Liquid/methods
  • Diterpenes/analysis
  • Euphorbia/chemistry
  • Plant Oils/chemistry
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization/methods
  • Spectrophotometry, Ultraviolet/methods

UNIGE affiliation entities

Citation (ISO format)

BICCHI, C. et al. HPLC-UV and HPLC-positive-ESI-MS analysis of the diterpenoid fraction from caper spurge (Euphorbia lathyris) seed oil. In: Phytochemical analysis, 2001, vol. 12, n° 4, p. 255–262. doi: 10.1002/pca.592

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0958-0344
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Technical informations

Creation

05/02/2010 14:32:00

First validation

05/02/2010 14:32:00

Update

14/03/2023 15:23:48

Status update

14/03/2023 15:23:47

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