en
Scientific article
English

Use of vancomycin silica stationary phase in packed capillary electrochromatography. II. Enantiomer separation of venlafaxine and O-desmethylvenlafaxine in human plasma

Published inJournal of chromatography, vol. 919, no. 1, p. 195-203
Publication date2001
Abstract

A capillary electrochromatography method, using vancomycin chiral stationary phase packed capillary, was optimized for the simultaneous chiral separation of the antidepressant drug venlafaxine and its main active metabolite O-desmethylvenlafaxine. Simultaneous baseline enantiomeric separation of the two compounds was obtained using a mobile phase composed of 100 mM ammonium acetate buffer pH 6/water/acetonitrile (5:5:90, v/v). The electrokinetic injection for sample introduction provided a limit of quantitation for both the compounds of 0.05 microg/ml racemate concentration suitable for the analysis of venlafaxine and metabolite in biological samples. The acetonitrile mobile phase concentration was found to modulate the analytes elution times, the enantiomeric resolution and the efficiency of the separation. The column was tested for repeatability and linearity showing RSD values (%) in the range of 0.13-0.24, 2.47-3.66 and 1.35-2.50 for migration time, sample/internal standard peak area ratio and enantiomeric resolution, respectively and correlation coefficients higher than 0.9990. The method was applied to the analysis of clinical samples of patients under depression therapy showing a stereoselective metabolism for venlafaxine.

Keywords
  • Antidepressive Agents, Second-Generation/blood/chemistry/isolation & purification
  • Chromatography, Micellar Electrokinetic Capillary/methods
  • Cyclohexanols/blood/chemistry/isolation & purification
  • Sensitivity and Specificity
  • Silicon Dioxide/chemistry
  • Stereoisomerism
  • Vancomycin/chemistry
Citation (ISO format)
FANALI, S. et al. Use of vancomycin silica stationary phase in packed capillary electrochromatography. II. Enantiomer separation of venlafaxine and O-desmethylvenlafaxine in human plasma. In: Journal of chromatography, 2001, vol. 919, n° 1, p. 195–203. doi: 10.1016/S0021-9673(01)00781-6
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Article (Published version)
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ISSN of the journal0021-9673
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