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Protein precipitation for the analysis of a drug cocktail in plasma by LC-ESI-MS

Published in Journal of pharmaceutical and biomedical analysis. 2004, vol. 35, no. 4, p. 913-20
Abstract Three protein precipitation (PP) procedures with acetonitrile (ACN), perchloric acid (PA) and trichloroacetic acid (TCA) were investigated for the analysis of a drug cocktail from human plasma samples containing three pharmaceutical compounds and their primary metabolites. For this purpose, a capillary liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) method was developed for the simultaneous analysis of the six tested compounds in less than 6 min. Matrix effect was tested for each PP procedure by means of a post-column infusion system. The three PP techniques were found effective in removing proteins from human plasma and were fully compatible with capillary LC-ESI-MS analysis. However, with acid precipitations, low analyte recovery and a high variability, probably due to analyte coprecipitation, were obtained. Finally, ACN was found to be the most effective PP technique with a recovery higher than 80% and CV inferior to 6%.
Keywords Blood Proteins/analysis/metabolismChemical PrecipitationChromatography, Liquid/methodsHumansSpectrometry, Mass, Electrospray Ionization/methods
PMID: 15193736
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FLEURY, Sandrine, RUDAZ, Serge, VEUTHEY, Jean-Luc. Protein precipitation for the analysis of a drug cocktail in plasma by LC-ESI-MS. In: Journal of pharmaceutical and biomedical analysis, 2004, vol. 35, n° 4, p. 913-20. doi: 10.1016/j.jpba.2004.03.005 https://archive-ouverte.unige.ch/unige:5092

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Deposited on : 2010-01-29

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