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Scientific Article
unige:5092 
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Protein precipitation for the analysis of a drug cocktail in plasma by LC-ESI-MS |
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| Published in | Journal of Pharmaceutical and Biomedical Analysis. 2004, vol. 35, no. 4, p. 913-20 | |
| Abstract | Three protein precipitation (PP) procedures with acetonitrile (ACN), perchloric acid (PA) and trichloroacetic acid (TCA) were investigated for the analysis of a drug cocktail from human plasma samples containing three pharmaceutical compounds and their primary metabolites. For this purpose, a capillary liquid chromatography-electrospray ionisation-mass spectrometry (LC-ESI-MS) method was developed for the simultaneous analysis of the six tested compounds in less than 6 min. Matrix effect was tested for each PP procedure by means of a post-column infusion system. The three PP techniques were found effective in removing proteins from human plasma and were fully compatible with capillary LC-ESI-MS analysis. However, with acid precipitations, low analyte recovery and a high variability, probably due to analyte coprecipitation, were obtained. Finally, ACN was found to be the most effective PP technique with a recovery higher than 80% and CV inferior to 6%. | |
| Keywords | Blood Proteins/analysis/metabolism — Chemical Precipitation — Chromatography, Liquid/methods — Humans — Spectrometry, Mass, Electrospray Ionization/methods | |
| Identifiers | PMID: 15193736 | |
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| Citation (ISO format) | SOUVERAIN, S., RUDAZ, Serge, VEUTHEY, Jean-Luc. Protein precipitation for the analysis of a drug cocktail in plasma by LC-ESI-MS. In: Journal of Pharmaceutical and Biomedical Analysis, 2004, vol. 35, n° 4, p. 913-20. https://archive-ouverte.unige.ch/unige:5092 |
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