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Scientific article
English

Ultrashort partial-filling technique in capillary electrophoresis for infinite resolution of tramadol enantiomers and its metabolites with highly sulfated cyclodextrins

Contributors

Rudaz, Serge; Le Saux, Thomas; Prat, Josiane; Gareil, Pierre; Veuthey, Jean-Luc

Published in

Electrophoresis, vol. 25, no. 16, p. 2761-2771

Publication date

2004

Abstract

The possibility to enhance resolution to infinite value in chiral capillary electrophoresis is attained as soon as the apparent mobility of one enantiomer becomes opposite to the other. This could be achieved on the basis of the carrier ability of multiple charged chiral selectors such as highly sulfated cyclodextrin (HS-CD). With tramadol and its phase I metabolites selected as model compounds, the HS-gamma-CD was found to be the most appropriate chiral selector. The CD concentration was determined where one enantiomer still migrated as a cation while the other migrated in the opposite side. Besides the chiral selector concentration, secondary parameters such as buffer concentration appeared to be critical to reach infinite resolution. The latter was achieved with partial filling technique using ultrashort separation zones (a few mm). In order to better understand the interaction mechanism between the selected CD and the analytes, the classical affinity capillary electrophoresis method, although not fully satisfactory because of ionic strength variations within a series of mobility shift measurements, was applied to estimate complexation constants and complex mobilities. The results obtained point to the prevailing role of complex mobility differences in the enantioselectivity mechanism.

Keywords

  • Buffers
  • Cyclodextrins/chemistry
  • Electrophoresis, Capillary/methods
  • Molecular Structure
  • Osmolar Concentration
  • Stereoisomerism
  • Temperature
  • Tramadol/chemistry/isolation & purification/metabolism
  • Viscosity

UNIGE affiliation entities

Citation (ISO format)

RUDAZ, Serge et al. Ultrashort partial-filling technique in capillary electrophoresis for infinite resolution of tramadol enantiomers and its metabolites with highly sulfated cyclodextrins. In: Electrophoresis, 2004, vol. 25, n° 16, p. 2761–2771. doi: 10.1002/elps.200406052

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0173-0835
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Technical informations

Creation

29/01/2010 14:28:00

First validation

29/01/2010 14:28:00

Update

14/03/2023 15:23:35

Status update

14/03/2023 15:23:34

Last indexation

18/03/2025 09:18:01
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