Use of large particles support for fast analysis of methadone and its primary metabolite in human plasma by liquid chromatography-mass spectrometry

Souverain, S.; Rudaz, Serge; Ortelli, Didier; Varesio, Emmanuel; Veuthey, Jean-Luc

doi:10.1016/S1570-0232(02)00783-3

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Title		Use of large particles support for fast analysis of methadone and its primary metabolite in human plasma by liquid chromatography-mass spectrometry
Authors		Souverain, S. Rudaz, Serge Ortelli, Didier Varesio, Emmanuel Veuthey, Jean-Luc
Published in		Journal of Chromatography. B. 2003, vol. 784, no. 1, p. 117-123
Abstract		A bioanalytical method was developed for the quantitation of methadone (MTD) and its primary metabolite, (EDDP) in plasma. The extraction step was performed within a capillary column packed with large particles (35x0.3 mm I.D.; d(p) 30 micrometer) at high flow-rate conditions (450 microliter/min). The separation was performed on a microbore analytical column (55x2 mm I.D.; d(p) 3 micrometer) coupled to a mass spectrometer (MS). This procedure was based on a column-switching unit. Analytes of interest were retained on the precolumn by hydrophobic interactions and backflushed from the precolumn to the analytical column. The detection was carried out with a MS single quadrupole equipped with an electrospray interface. The total analysis time was 6 min. The limits of quantification were evaluated at 10 and 25 ng/ml for MTD and EDDP, respectively. At this level, good accuracies were obtained for both analytes with repeatability values less than 18%.
Keywords		Chromatography, Liquid/methods — Humans — Mass Spectrometry/methods — Methadone/blood
Identifiers		DOI: 10.1016/S1570-0232(02)00783-3 PMID: 12504189
Full text		Article (Published version) (284 Kb) - Limited access to UNIGE
Structures		Faculté des sciences / Section des sciences pharmaceutiques
Citation (ISO format)		SOUVERAIN, S. et al. Use of large particles support for fast analysis of methadone and its primary metabolite in human plasma by liquid chromatography-mass spectrometry. In: Journal of Chromatography. B, 2003, vol. 784, n° 1, p. 117-123. doi: 10.1016/S1570-0232(02)00783-3 https://archive-ouverte.unige.ch/unige:5083

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Deposited on : 2010-01-29

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Use of large particles support for fast analysis of methadone and its primary metabolite in human plasma by liquid chromatography-mass spectrometry