en
Scientific article
English

Use of large particles support for fast analysis of methadone and its primary metabolite in human plasma by liquid chromatography-mass spectrometry

Published inJournal of chromatography. B, vol. 784, no. 1, p. 117-123
Publication date2003
Abstract

A bioanalytical method was developed for the quantitation of methadone (MTD) and its primary metabolite, (EDDP) in plasma. The extraction step was performed within a capillary column packed with large particles (35x0.3 mm I.D.; d(p) 30 micrometer) at high flow-rate conditions (450 microliter/min). The separation was performed on a microbore analytical column (55x2 mm I.D.; d(p) 3 micrometer) coupled to a mass spectrometer (MS). This procedure was based on a column-switching unit. Analytes of interest were retained on the precolumn by hydrophobic interactions and backflushed from the precolumn to the analytical column. The detection was carried out with a MS single quadrupole equipped with an electrospray interface. The total analysis time was 6 min. The limits of quantification were evaluated at 10 and 25 ng/ml for MTD and EDDP, respectively. At this level, good accuracies were obtained for both analytes with repeatability values less than 18%.

Keywords
  • Chromatography, Liquid/methods
  • Humans
  • Mass Spectrometry/methods
  • Methadone/blood
Citation (ISO format)
SOUVERAIN, S. et al. Use of large particles support for fast analysis of methadone and its primary metabolite in human plasma by liquid chromatography-mass spectrometry. In: Journal of chromatography. B, 2003, vol. 784, n° 1, p. 117–123. doi: 10.1016/S1570-0232(02)00783-3
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ISSN of the journal1570-0232
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