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Title

Simultaneous stereoselective analysis of tramadol and its main phase I metabolites by on-line capillary zone electrophoresis-electrospray ionization mass spectrometry

Authors
Fanali, Salvatore
Published in Journal of Chromatography. A. 2000, vol. 868, no. 2, p. 295-303
Abstract On-line combination of partial filling capillary electrophoresis and electrospray ionization mass spectrometry was demonstrated for the simultaneous enantioseparation of tramadol and its main phase I metabolites. The partial filling technique was efficient at avoiding MS contamination by the chiral selector. Different experimental factors were investigated, including the chiral selector nature and concentration, plug length as well as the separation temperature. The best enantioseparation of the investigated compounds was achieved with a coated polyvinyl alcohol capillary and a 40 mM ammonium acetate buffer, pH 4.0, adding sulfobutyl ether beta-cyclodextrin (2.5 mg/ml) as the chiral selector. The charged cyclodextrin not only allowed enantioseparation of tramadol and its metabolites, but also improved the selectivity of compounds with the same molecular mass. Finally, CE-electrospray ionisation-MS was successfully applied to the stereoselective analysis of tramadol and its main metabolites in plasma after a simple liquid-liquid extraction.
Keywords Electrophoresis, Capillary/methodsHumansMass Spectrometry/methodsTramadol/analysis/metabolism
Identifiers
PMID: 10701679
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RUDAZ, Serge et al. Simultaneous stereoselective analysis of tramadol and its main phase I metabolites by on-line capillary zone electrophoresis-electrospray ionization mass spectrometry. In: Journal of Chromatography. A, 2000, vol. 868, n° 2, p. 295-303. https://archive-ouverte.unige.ch/unige:5072

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Deposited on : 2010-01-28

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