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Experimental design in supercritical fluid extraction of cocaine from coca leaves

Brachet, Anne
Gauvrit, Jean-Yves
Longeray, Rémi
Lantéri, Pierre
Published in Journal of biochemical and biophysical methods. 2000, vol. 43, no. 1-3, p. 353-66
Abstract An optimisation procedure for the supercritical fluid extraction (SFE) of cocaine from the leaves of Erythroxylum coca var. coca was investigated by means of experimental design. After preliminary experiments where the SFE rate-controlling mechanism was determined, a central composite design was applied to evaluate interactions between selected SFE factors such as pressure, temperature, nature and percentage of the polar modifier, as well as to optimise these factors. Predicted and experimental contents of cocaine were compared and robustness of the extraction method estimated by drawing response surfaces. The analysis of cocaine in crude extracts was carried out by capillary GC equipped with a flame ionisation detector (GC-FID), as well as by capillary GC coupled with a mass spectrometer (GC-MS) for peak identification.
Keywords Cacao/chemistryCarbon Dioxide/chemistryChromatography/methodsChromatography, Gas/methodsCocaine/isolation & purificationGas Chromatography-Mass SpectrometryKineticsModels, StatisticalPressureSoftwareTemperatureTime Factors
PMID: 10869687
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BRACHET, Anne et al. Experimental design in supercritical fluid extraction of cocaine from coca leaves. In: Journal of biochemical and biophysical methods, 2000, vol. 43, n° 1-3, p. 353-66. doi: 10.1016/S0165-022X(00)00062-2 https://archive-ouverte.unige.ch/unige:5069

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Deposited on : 2010-01-28

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