UNIGE document Scientific Article
previous document  unige:4868  next document
add to browser collection

Fast chiral separation of drugs using columns packed with sub-2 mum particles and ultra-high pressure

Published in Chirality. 2009, vol. 22, no. 3, p. 320-330
Abstract The use of columns packed with sub-2 mum particles in liquid chromatography with very high pressure conditions (known as UHPLC) was investigated for the fast enantioseparation of drugs. Two different procedures were evaluated and compared using amphetamine derivatives and beta-blockers as model compounds. In one case, cyclodextrins (CD) were directly added to the mobile phase and chiral separations were carried out in less than 5 min. However, this strategy suffered from several drawbacks linked to column lifetime and low chromatographic efficiencies. In the other case, the analysis of enantiomers was carried out after a derivatization procedure using two different reagents, 2,3,4-tri-O-acetyl-alpha-D-arabinopyranosyl isothiocyanate (AITC) and N-alpha-(2,4-dinitro-5-fluorophenyl)-L-alaninamide (Marfey's reagent). Separation of several amphetamine derivatives contained within the same sample was achieved in 2-5 min with high efficiency and selectivity. The proposed approach was also successfully applied to the enantiomeric purity determination of (+)-(S)-amphetamine and (+)-(S)-methamphetamine. Similar results were obtained with beta-blockers, and the separation of 10 enantiomers was carried out in less than 3 min, whereas the individual separation of several beta-blocker enantiomers was performed in 1 min or less. Chirality, 2010. (c) 2009 Wiley-Liss, Inc.
Keywords Fast-LCChiral separationEnantioseparationUHPLCUPLCSub-2 m
PMID: 19544347
Full text
Article (Published version) (333 Kb) - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
GUILLARME, Davy et al. Fast chiral separation of drugs using columns packed with sub-2 mum particles and ultra-high pressure. In: Chirality, 2009, vol. 22, n° 3, p. 320-330. doi: 10.1002/chir.20745 https://archive-ouverte.unige.ch/unige:4868

482 hits

1 download


Deposited on : 2010-01-25

Export document
Format :
Citation style :