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Title

CD4-independent protective cytotoxic T cells induced in early life by a non-replicative delivery system based on virus-like particles

Authors
Leclerc, Claude
Published in Virology. 2003, vol. 305, no. 2, p. 428-35
Abstract The relative immaturity of the neonatal immune system limits CD4(+) Th1 and cytotoxic T lymphocyte (CTL) responses, and represents a significant challenge for the development of vaccines against intracellular pathogens. In this report, we demonstrate the ability of a non-replicative delivery system based on parvovirus-like particles (VLP) to induce CTL responses in the neonatal period. A single immunization of 1-week-old BALB/c mice with recombinant VLP carrying a CD8(+) T cell determinant from lymphocytic choriomeningitis virus (VLP-LCMV) induced antigen-specific CD8(+) cytotoxic T cells that were similar to those elicited by adult immunization, as assessed by cytotoxic activity, interferon (IFN)-gamma secretion, cytotoxic precursor cell frequencies, in vitro avidity for antigen and protective activity against viral challenge. These CTL responses are elicited within 2 weeks of a single immunization, in the absence of adjuvant and independently of the presence and help of CD4(+) T cells, highlighting the potential of VLP as candidate vaccine vectors in early life.
Keywords Age FactorsAmino Acid SequenceAnimalsAnimals, NewbornAntigens, CD4/physiologyImmunizationInterferon-gamma/biosynthesisLymphocytic choriomeningitis virus/immunologyMiceMice, Inbred BALB CMolecular Sequence DataT-Lymphocytes, Cytotoxic/immunologyViral Vaccines/immunologyVirion/immunology
Identifiers
PMID: 12573588
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Article (Published version) (121 Kb) - public document Free access
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Research group Centre de Vaccinologie et d'Immunologie néonatale (177)
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MARTINEZ, Javier et al. CD4-independent protective cytotoxic T cells induced in early life by a non-replicative delivery system based on virus-like particles. In: Virology, 2003, vol. 305, n° 2, p. 428-35. https://archive-ouverte.unige.ch/unige:47578

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Deposited on : 2015-03-04

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