CD4-independent protective cytotoxic T cells induced in early life by a non-replicative delivery system based on virus-like particles

Article Article scientifique Article CD4-independent protective cytotoxic T cells induced in early life by a non-replicative delivery system based on virus-like particles eng 2003 author Martinez Javier 87672 author Regner Matthias 133794 author Kovarik Jiri 114989 author Zarei Haller Shohreh 514117 author Hauser Conrad 22348 author Lambert Paul Henri 25121 author Leclerc Claude author Siegrist Claire-Anne 26816 428-435 10.1006/viro.2002.1775 12573588 0042-6822 The relative immaturity of the neonatal immune system limits CD4(+) Th1 and cytotoxic T lymphocyte (CTL) responses, and represents a significant challenge for the development of vaccines against intracellular pathogens. In this report, we demonstrate the ability of a non-replicative delivery system based on parvovirus-like particles (VLP) to induce CTL responses in the neonatal period. A single immunization of 1-week-old BALB/c mice with recombinant VLP carrying a CD8(+) T cell determinant from lymphocytic choriomeningitis virus (VLP-LCMV) induced antigen-specific CD8(+) cytotoxic T cells that were similar to those elicited by adult immunization, as assessed by cytotoxic activity, interferon (IFN)-gamma secretion, cytotoxic precursor cell frequencies, in vitro avidity for antigen and protective activity against viral challenge. These CTL responses are elicited within 2 weeks of a single immunization, in the absence of adjuvant and independently of the presence and help of CD4(+) T cells, highlighting the potential of VLP as candidate vaccine vectors in early life. Age Factors Amino Acid Sequence Animals Animals, Newborn Antigens, CD4/physiology Immunization Interferon-gamma/biosynthesis Lymphocytic choriomeningitis virus/immunology Mice Mice, Inbred BALB C Molecular Sequence Data T-Lymphocytes, Cytotoxic/immunology Viral Vaccines/immunology Virion/immunology Dewey 616.07 Dewey 618 137 Département de pathologie et immunologie 21F 58 Département de pédiatrie, gynécologie et obstétrique 223 false 143 Centre de Vaccinologie et d'Immunologie néonatale 177 Virology 305 2 f5ac9dd5-f744-434f-a05b-1601697828b5 Article (Published version) Public 1-s2.0-S0042682202917757-main.pdf 124405 application/pdf openAccess Faculté de médecine / Section de médecine fondamentale / Département de pathologie et immunologie Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

Title	Published in	Access level	OA Policy	Year	Views	Downloads

CD4-independent protective cytotoxic T cells induced in early life by a non-replicative delivery system based on virus-like particles	Virology			2003	621	501

Archive ouverte UNIGE

Zarei Haller, Shohreh