Dynamics of sub-lethal effects of nano-CuO on the microalga Chlamydomonas reinhardtii during short-term exposure

Von Moos, Nadia Rachel; Maillard, Luca; Slaveykova, Vera

doi:10.1016/j.aquatox.2015.02.010

Scientific article

English

Dynamics of sub-lethal effects of nano-CuO on the microalga Chlamydomonas reinhardtii during short-term exposure

Contributors

Von Moos, Nadia Rachel; Maillard, Luca; Slaveykova, Vera

Published in

Aquatic toxicology, vol. 161, p. 267-275

Publication date

2015

Abstract

Though nano-CuO has been classified as toxic toward aquatic microorganisms and its use in variousapplications is expected to increase in near future, its ecotoxicity is currently poorly understood. Theaim of this study was to investigate the mechanisms of nano-CuO toxicity based on the paradigm ofoxidative stress, the dynamics of response over 24 h, and the modulating effect of exposure conditionson toxicity and responses. To this end, the model microalga Chlamydomonas reinhardtii was exposed to10 mg L−1nano-CuO in five different exposure media, including two different growth media, two Good'sbuffers, and natural lake water. The measured endpoints included cell growth, morphological aspect,chlorophyll autofluorescence, oxidative stress, and membrane permeabilization. The results suggest thatagglomerated nano-CuO is toxic and that exposure media are decisive in whether or not particles or freeCu ions are the main mediators of toxicity. A significant particle effect was only observed in the Good'sbuffer 3-(N-morpholino) propanesulfonic acid. However, nano-CuO particles especially influenced thedynamics of response early in exposure, between 0 h and 5 h, suggesting that an adaptation to particlestress may occur or that particles modify the bioavailability of the free Cu ions in early exposure.

Keywords

Engineered nanoparticles
(Nano-)ecotoxicology
Reactive oxygen species
Aquatic microorganisms
Flow cytometry

UNIGE affiliation entities

Faculté des sciences / Section des sciences de la Terre et de l'Environnement / Département F.-A. Forel des sciences de l'environnement et de l'eau

Citation (ISO format)

VON MOOS, Nadia Rachel, MAILLARD, Luca, SLAVEYKOVA, Vera. Dynamics of sub-lethal effects of nano-CuO on the microalga Chlamydomonas reinhardtii during short-term exposure. In: Aquatic toxicology, 2015, vol. 161, p. 267–275. doi: 10.1016/j.aquatox.2015.02.010

Article (Published version)

Identifiers

PID : unige:47548
DOI : 10.1016/j.aquatox.2015.02.010

Additional URL for this publication

http://linkinghub.elsevier.com/retrieve/pii/S0166445X15000612

Journal ISSN

0166-445X

696views

9downloads

Creation

03/03/2015 10:01:00

First validation

03/03/2015 10:01:00

Update

14/03/2023 22:57:32

Status update

14/03/2023 22:57:32

Last indexation

30/10/2024 23:13:57

Dynamics of sub-lethal effects of nano-CuO on the microalga Chlamydomonas reinhardtii during short-term exposure

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation