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Title

High resolution mini-two-dimensional gel electrophoresis automatically run and stained in less than six hours with small, ready to use slab gels

Authors
Augsburger, Valérie
Published in Clinical Chemistry. 1988, vol. 34, no. 1, p. 166-170
Abstract Although two-dimensional (2-D) gel electrophoresis is one of the most powerful techniques for analyzing protein mixtures, SIts application in routine clinical laboratories is currently limited, because it is time-consuming, complex, and relatively expensive. Here we describe a method for automatically running and staining “high-resolution” mini 2-0 electrophoresis gels in <6 h, by using “ready-to-use” slab gels and a PhastSystem#{e1l7e4c}trophoresisapparatus. We present 2-0 gel electrophoretograms of 25 nL of plasma, as well as their automatic computer analysis. For comparison, a conventional 2-D gel electrophoresis profile of 200 nL of a plasma sample is shown. The technique is easy to perform, highly sensitive, rapid, and potentially useful in semi-routine clinical chemistrylaboratories.
Keywords proteinsisoelectric focusingcomputerized gelevaluationSDS-PAGE
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Research groups Computer Vision and Multimedia Laboratory
Multimodal Interaction Group
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HOCHSTRASSER, Denis et al. High resolution mini-two-dimensional gel electrophoresis automatically run and stained in less than six hours with small, ready to use slab gels. In: Clinical Chemistry, 1988, vol. 34, n° 1, p. 166-170. https://archive-ouverte.unige.ch/unige:47447

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Deposited on : 2015-03-03

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