High resolution mini-two-dimensional gel electrophoresis automatically run and stained in less than six hours with small, ready to use slab gels

Article Article scientifique Article High resolution mini-two-dimensional gel electrophoresis automatically run and stained in less than six hours with small, ready to use slab gels eng 1988 author Hochstrasser Denis 26422 author Augsburger Valérie author Pun Thierry 22776 author Weber Dominique 60229 author Pellegrini Christian 24713 author Muller Alex-F. 24992 166-170 0009-9147 http://www.clinchem.org/content/34/1/166.full.pdf+html Although two-dimensional (2-D) gel electrophoresis is one of the most powerful techniques for analyzing protein mixtures, SIts application in routine clinical laboratories is currently limited, because it is time-consuming, complex, and relatively expensive. Here we describe a method for automatically running and staining “high-resolution” mini 2-0 electrophoresis gels in <6 h, by using “ready-to-use” slab gels and a PhastSystem#{e1l7e4c}trophoresisapparatus. We present 2-0 gel electrophoretograms of 25 nL of plasma, as well as their automatic computer analysis. For comparison, a conventional 2-D gel electrophoresis profile of 200 nL of a plasma sample is shown. The technique is easy to perform, highly sensitive, rapid, and potentially useful in semi-routine clinical chemistrylaboratories. proteins isoelectric focusing computerized gel evaluation SDS-PAGE Dewey 025.063 13 Centre universitaire d'informatique 0D3 32 Département d'informatique 18 false 895 Computer Vision and Multimedia Laboratory 725 Multimodal Interaction Group Clinical chemistry 34 1 f822042a-cab7-47e7-84fe-d47077c281c9 Article (Published version) Public 1988Hochstrasser_high_resol.pdf 991566 application/pdf openAccess Centres et instituts / Centre universitaire d'informatique Faculté des sciences / Département d'informatique

Title	Published in	Access level	OA Policy	Year	Views	Downloads

High resolution mini-two-dimensional gel electrophoresis automatically run and stained in less than six hours with small, ready to use slab gels	Clinical chemistry			1988	543	917

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Muller, Alex-F.